A method for the determination of binding constants by electrospray ionization mass spectrometry

被引：143
|
作者：
Kempen, EC [1 ]
Brodbelt, JS [1 ]
机构：
[1] Univ Texas, Dept Chem & Biochem, Austin, TX 78712 USA
来源：
ANALYTICAL CHEMISTRY | 2000年 / 72卷 / 21期
关键词：
D O I：
10.1021/ac000540e
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
A new method for the determination of binding constants using electrospray ionization mass spectrometry is presented. The intensity of a reference complex with a known log K value is monitored before and after addition of a second host or guest. On the basis of the change in intensity of the reference complex and extrapolation from a calibration curve, the log K value is then derived for the complex of interest using a set of simultaneous equilibrium equations. Binding constants of several crown ether-alkali metal cation complexes that were previously studied were determined to validate this strategy. Log K values for complexes involving dibenzo-16-crown-5 and its sym-oxyacetate derivative with Na+ or K+ were also determined.
引用
收藏
页码：5411 / 5416
页数：6
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