DNA of Drosophila melanogaster contains 5-methylcytosine

被引：148
|
作者：
Gowher, H
Leismann, O
Jeltsch, A
机构：
[1] Inst Biochem, Fachbereich 8, D-35392 Giessen, Germany
[2] Univ Bayreuth, Lehrstuhl Genet, D-95440 Bayreuth, Germany
来源：
EMBO JOURNAL | 2000年 / 19卷 / 24期
关键词：
DNA methylation; DNA methyltransferase; DNA modification; Drosophila melanogaster; 5-methylcytosine;
D O I：
10.1093/emboj/19.24.6918
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
It is commonly accepted that the DNA of Drosophila melanogaster does not contain 5-methylcytosine, which is essential in the development of most eukaryotes. We have developed a new, highly specific and sensitive assay to detect the presence of 5-methylcytosine in genomic DNA. The DNA is degraded to nucleosides, 5-methylcytosine purified by HPLC and, for detection by 1D- and 2D-TLC, radiolabeled using deoxynucleoside kinase and [gamma-P-32]ATP. Using this assay, we show here that 5-methylcytosine occurs in the DNA of D.melanogaster at a level of similar to1 in 1000-2000 cytosine residues in adult flies. DNA methylation is detectable in all stages of D.melanogaster development.
引用
收藏
页码：6918 / 6923
页数：6
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