Cutting Edge Genetics: CRISPR/Cas9 Editing of Plant Genomes

被引：31
|
作者：
Soyars, Cara L.
Peterson, Brenda A.
Burr, Christian A.
Nimchuk, Zachary L. [1 ]
机构：
[1] Univ N Carolina, Dept Biol, 4155 Genome Sci Bldg,250 Bell Tower Dr, Chapel Hill, NC 27599 USA
来源：
PLANT AND CELL PHYSIOLOGY | 2018年 / 59卷 / 08期
基金：
美国国家科学基金会;
关键词：
Cas9; CRISPR; Genome engineering; Off-target; RNA-GUIDED ENDONUCLEASE; TARGETED MUTAGENESIS; HIGH-EFFICIENCY; TRANSCRIPTIONAL REGULATION; HOMOLOGOUS RECOMBINATION; LATER GENERATIONS; ANALYSIS REVEALS; PAIRED NICKASES; DNA CLEAVAGE; HUMAN-CELLS;
D O I：
10.1093/pcp/pcy079
中图分类号：
Q94 [植物学];
学科分类号：
071001 ;
摘要：
The clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated nuclease 9 (Cas9) system is a genome editing technology transforming the field of plant biology by virtue of the system's efficiency and specificity. The system has quickly evolved for many diverse applications including multiplex gene mutation, gene replacement and transcriptional control. As CRISPR/Cas9 is increasingly applied to plants, it is becoming clear that each component of the system can be modified to improve editing results. This review aims to highlight common considerations and options when conducting CRISPR/Cas9 experiments.
引用
收藏
页码：1608 / 1620
页数：13
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