Determination of Methadone in Biological Samples Using Liquid Phase Microextraction with Back Extraction Combined with LC

被引：10
|
作者：
Ebrahimzadeh, Homeira [1 ]
Khezri, Ehsan [1 ]
Kamarei, Fahimeh [1 ]
机构：
[1] Shahid Beheshti Univ GC, Dept Chem, Tehran, Iran
来源：
CHROMATOGRAPHIA | 2010年 / 72卷 / 3-4期
关键词：
Column liquid chromatography; Liquid phase microextraction with back extraction; Methadone in plasma and urine; CHROMATOGRAPHY-MASS SPECTROMETRY; HUMAN PLASMA; QUANTITATIVE-DETERMINATION; GAS-CHROMATOGRAPHY; PRIMARY METABOLITE; AROMATIC-AMINES; WHOLE-BLOOD; BREAST-MILK; WATER; FLUIDS;
D O I：
10.1365/s10337-010-1636-4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Liquid phase microextraction with back extraction (LPME-BE) combined with liquid chromatography-ultra violet (LC-UV) was applied for the extraction and determination of methadone in biological fluids. At the optimized conditions, an enrichment factor of 386 and detection limit (LOD) of 0.2 mu g L(-1) were obtained. The calibration curve was linear (r (2) = 0.989) in the concentration range of 0.6-1,000 mu g L(-1). Within-day relative standard deviation RSD (S/N = 3) and between-day RSD were 2.7 and 5.9%, respectively. The feasibility of the proposed method was evaluated by extraction and determination of methadone in plasma and urine samples and satisfactory results were obtained.
引用
收藏
页码：231 / 238
页数：8
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