Monitoring base excision repair proteins on damaged DNA using human cell extracts

被引：10
|
作者：
Parsons, JL [1 ]
Dianov, GL [1 ]
机构：
[1] MRC, Radiat & Genome Stabil Unit, Didcot OX11 0RD, Oxon, England
来源：
BIOCHEMICAL SOCIETY TRANSACTIONS | 2004年 / 32卷
关键词：
base excision repair; DNA polymerase beta; DNA repair; X-ray cross-complementing protein 1 (XRCC1);
D O I：
10.1042/BST0320962
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
BER (base excision repair) is a major pathway for the removal of simple lesions in DNA including base damage and base loss (abasic site). we have developed an assay, using formaldehyde cross-linking during repair in human cell extracts, to observe BER proteins involved in the repair of damaged DNA. This approach allows visualization of repair proteins on damaged DNA during BER in human cell extracts and provides a detailed view of the molecular events leading to repair.
引用
收藏
页码：962 / 963
页数：2
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