Protocol to Generate Senescent Cells from the Mouse Hepatic Cell Line AML12 to Study Hepatic Aging

被引：8

作者：

Tripathi, Madhulika ^{[1
]}

Yen, Paul Michael ^{[1
,2
]}

Singh, Brijesh Kumar ^{[1
]}

机构：

[1] Duke NUS Med Sch, Cardiovasc & Metab Disorders Program, Singapore 169857, Singapore

[2] Duke Univ, Sch Med, Durham, NC USA

来源：

STAR PROTOCOLS | 2020年 / 1卷 / 02期

基金：

英国医学研究理事会;

关键词：

MARKER;

D O I：

10.1016/j.xpro.2020.100064

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Previously developed senescent primary fibroblast models have limited rele-vance to study age-related changes in metabolically active tissues such as the liver. Here, we describe a protocol to generate senescent cells from the mouse hepatic cell line, AML12. These senescent cells exhibit molecular and metabolic signatures that are similar to those observed in livers from aged mice. These se-nescent AML12 cells should be a useful in vitro model to study the metabolic ef-fects of aging in the liver. For complete details on the use and execution of this protocol, please refer to Singh et al. (2020).

引用

页数：10

共 32 条

[21] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers
Takamichi Ishii
Kentaro Yasuchika
Ken Fukumitsu
Tatsuya Kawamoto
Miho Kawamura-Saitoh
Yuji Amagai
Iwao Ikai
Shinji Uemoto
Eihachiro Kawase
Hirofumi Suemori
Norio Nakatsuji
Cell and Tissue Research, 2010, 339 : 505 - 512
[22] In vitro hepatic maturation of human embryonic stem cells by using a mesenchymal cell line derived from murine fetal livers
Ishii, Takamichi
Yasuchika, Kentaro
Fukumitsu, Ken
Kawamoto, Tatsuya
Kawamura-Saitoh, Miho
Amagai, Yuji
Ikai, Iwao
Uemoto, Shinji
Kawase, Eihachiro
Suemori, Hirofumi
Nakatsuji, Norio
CELL AND TISSUE RESEARCH, 2010, 339 (03) : 505 - 512
[23] Protocol to study ex vivo T cell priming by conventional dendritic cells from the mouse spleen
Ashayeripanah, Mitra
Villadangos, Jose A.
STAR PROTOCOLS, 2024, 5 (04):
[24] Development of a liver-specific marker transgenic mouse for the study of alternative pathway of hepatic regeneration from bone marrow cells
Garcia-Bravo, M.
Fernandez, A.
Chinchon, R.
Angel Martin, M.
Rubio, N.
Blanco, J.
Montoliu, L.
Segovia, J. C.
HUMAN GENE THERAPY, 2013, 24 (12) : A104 - A105
[25] Efficient Generation of Hepatic Cells from Multipotent Adult Mouse Germ-Line Stem Cells Using an OP9 Co-Culture System
Streckfuss-Boemeke, Katrin
Jende, Joerg
Cheng, I-Fen
Hasenfuss, Gerd
Guan, Kaomei
CELLULAR REPROGRAMMING, 2014, 16 (01) : 65 - 76
[26] A novel differentiation factor for PC12 cells from culture supernatant of mouse hepatocyte cell line MLE-15A2
Ihara, S
Nishikawa, T
Kimura, K
Fujiyoshi, T
Shirai, T
Komi, A
Kanda, H
Yamori, T
Fukui, Y
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 1996, 60 (08) : 1339 - 1345
[27] An immortalized drug-resistant cell line established from 12-13-day mouse embryos for the propagation of human embryonic stem cells
Iuchi, S
Marsch-Moreno, M
Velez-DelValle, C
Easley, K
Harcuch, WK
Green, H
DIFFERENTIATION, 2006, 74 (04) : 160 - 166
[28] Signals from pancreatic mesoderm influence the expression of a pancreatic phenotype in hepatic stem-like cell line PHeSC-A2 in vitro: A preliminary study
Augustine, Tanya N.
Kramer, Beverley
ACTA HISTOCHEMICA, 2011, 113 (03) : 349 - 352
[29] Hepatic sdf-1 (CXCL12) acts downstream of VEGF to recruit liver sinusoidal endothelial cell progenitor cells from the bone marrow in liver regeneration
DeLeve, Laurie D.
Wang, Xiangdong
Wang, Lei
Gaarde, William A.
HEPATOLOGY, 2013, 58 : 282A - 282A
[30] Preparation, characterization, and computational study on transition metal complexes derived from thiophene Schiff base and evaluation of their cytotoxicity against hepatic cell line, antioxidant activities, and ligand's anticorrosive properties
Sayed, Fatma N.
Ashmawy, Ashraf M.
Mohamed, Gehad G.
Deghadi, Reem G.
APPLIED ORGANOMETALLIC CHEMISTRY, 2024, 38 (04)

← 1 2 3 4 →