Evaluation of genetic diversity among Jatropha curcas (L.) by RAPD analysis

被引:0
|
作者
Subramanyam, K. [2 ]
Rao, Dowlathabad Muralidhara [1 ]
Devanna, N. [3 ]
Aravinda, A. [2 ]
Pandurangadu, V. [2 ]
机构
[1] Sri Krishnadevaraya Univ, Dept Biotechnol, Anantapur 515001, Andhra Pradesh, India
[2] Jawaharlal Nehru Technol Univ, Oil Technol Res Inst, Dept Biochem, Anantapur 515001, Andhra Pradesh, India
[3] Jawaharlal Nehru Technol Univ, Dept Chem, Anantapur 515001, Andhra Pradesh, India
来源
INDIAN JOURNAL OF BIOTECHNOLOGY | 2010年 / 9卷 / 03期
关键词
Cluster analysis; DNA fingerprinting; genetic diversity; Jatropha curcas; PCR; principal component analysis; RAPD; MARKERS; ISSR;
D O I
暂无
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The genetic diversity and pedigree analysis of Jatropha curcas (L.) was evaluated through RAPD markers. A total number of 10 accessions, collected from different ecoclimatic zones of India, were screened with 43 random decamer primers to evaluate polymorphism. Selected 10 primers generated 125 bands, 76 of which were found to be polymorphic. The amplification products ranged from 9-14 bands for different primers. Each primer produced on an average 12.5 bands per primer, of which 7.6 were polymorphic. UPGMA method was used to construct dendrogram and genetic distance matrix was determined using squared Euclidean distances. Cluster analysis of data using UPGMA algorithm placed the ten accessions into two main clusters, the genetic dissimilarity matrix between genotypes ranged from 0-8 and the principal component analysis placed the 10 accessions into three groups.
引用
收藏
页码:283 / 288
页数:6
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