Construction and characterization of a cDNA expression library from the endangered Hu sheep

被引：6
|
作者：
Hu, P. -F. [1 ,2 ]
Li, X. -C. [1 ]
Liu, H. -K. [1 ]
Guan, W. -J. [1 ]
Ma, Y. -H. [1 ]
机构：
[1] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100193, Peoples R China
[2] Chinese Acad Agr Sci, Inst Special Econ Anim & Plant Sci, Beijing 100193, Peoples R China
来源：
GENETICS AND MOLECULAR RESEARCH | 2014年 / 13卷 / 04期
关键词：
Hu sheep; cDNA expression library; Endangered animals; MOLECULAR CHARACTERIZATION; TISSUE EXPRESSION; CLONES; GENE;
D O I：
10.4238/2014.October.31.16
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Hu sheep is one of the most important species in China; it is also listed as one of the 78 nationally protected domestic animals by the Chinese government in 2000. The construction of cDNA expression library of Hu sheep is of great significance for protecting individual genomes, generating transgenic sheep, and conducting clinical research using cDNA from Hu sheep. In this study, the total RNA from the ear tissue of Hu sheep was extracted, and a cDNA expression library was constructed using the SMART (TM) technique. The titer of amplified cDNA library was 1.09 x 10(10) PFU/mL, the rate of recombination was above 91.6%, and the average size of fragments was 1.1 kb. This study has an important significance for the preservation of Hu sheep resources at the genome level.
引用
收藏
页码：9019 / 9023
页数：5
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