Development and fit-for-purpose validation of a LC-MS/MS assay for fibrinogen peptide A quantitation in human plasma

被引：5

作者：

Lin, Mingxiang ^{[1
]}

Lassman, Michael E. ^{[1
]}

Weiner, Russell ^{[1
]}

Laterza, Omar F. ^{[1
]}

机构：

[1] Merck Res Labs, Clin Dev Lab, Rahway, NJ 07065 USA

来源：

BIOANALYSIS | 2014年 / 6卷 / 13期

关键词：

D O I：

10.4155/bio.14.148

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Background: Fibrinopeptide A (FPA) is a plasma peptide, formed by the action of thrombin on fibrinogen during clog formation. FPA represents a direct indicator of thrombin activity and could potentially be used as a biomarker for anti-thrombotic therapy development. Results/Methodology: A LC-MS/MS assay with a high throughput solid phase extraction procedure was developed and validated to measure FPA in plasma. The lower limit-of-quantitation (LLOQ) of this assay was determined to be 0.16 nM. The inter- and intra-day%CV was <15%. Freeze-thaw stability of FPA was +/- 30% up to 3 cycles and linear response of FPA was observed for plasma dilution up to 16-fold. Conclusion: The assay was validated and the biological variability of FPA in plasma was established (1-30 nM).

引用

页码：1759 / 1766

页数：8

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