Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins

被引：34

作者：

Knöpfel, T ^{[1
]}

Tomita, K ^{[1
]}

Shimazaki, R ^{[1
]}

Sakai, R ^{[1
]}

机构：

[1] RIKEN, Brain Sci Inst, Lab Neuronal Circuit Dynam, Wako, Saitama 3510198, Japan

来源：

METHODS | 2003年 / 30卷 / 01期

关键词：

green fluorescent protein; optical imaging; voltage-sensitive dyes; biosensors; fluorescence resonance energy transfer;

D O I：

10.1016/S1046-2023(03)00006-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Optical imaging of electrical activity using voltage-sensitive dyes has been envisaged for many years as a powerful method to investigate multineuronal representation of information processing in brain tissue. This article describes the advent of novel genetically targeted voltage-sensitive fluorescent proteins. This new class of membrane voltage sensors overcomes previous limitations related to the nonselective staining of membranes associated with conventional voltage-sensitive dyes. Here, we discuss the methodology, applications, and potential advantages of this novel technique. (C) 2003 Elsevier Science (USA). All rights reserved.

引用

页码：42 / 48

页数：7

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