Optical recordings of membrane potential using genetically targeted voltage-sensitive fluorescent proteins

被引：34
|
作者：
Knöpfel, T [1 ]
Tomita, K [1 ]
Shimazaki, R [1 ]
Sakai, R [1 ]
机构：
[1] RIKEN, Brain Sci Inst, Lab Neuronal Circuit Dynam, Wako, Saitama 3510198, Japan
来源：
METHODS | 2003年 / 30卷 / 01期
关键词：
green fluorescent protein; optical imaging; voltage-sensitive dyes; biosensors; fluorescence resonance energy transfer;
D O I：
10.1016/S1046-2023(03)00006-9
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Optical imaging of electrical activity using voltage-sensitive dyes has been envisaged for many years as a powerful method to investigate multineuronal representation of information processing in brain tissue. This article describes the advent of novel genetically targeted voltage-sensitive fluorescent proteins. This new class of membrane voltage sensors overcomes previous limitations related to the nonselective staining of membranes associated with conventional voltage-sensitive dyes. Here, we discuss the methodology, applications, and potential advantages of this novel technique. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：42 / 48
页数：7
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