Biocomponents from Opuntia robusta and Opuntia streptacantha fruits protect against diclofenac-induced acute liver damage in vivo and in vitro

被引：7
|
作者：
Stephanie Villa-Jaimes, Gloria [1 ]
Alejandro Aguilar-Mora, Fabio [2 ]
Antonio Gonzalez-Ponce, Herson [1 ,2 ]
Javier Avelar-Gonzalez, Francisco [3 ]
Martinez Saldana, Ma Consolacion [1 ]
Buist-Homan, Manon [3 ,4 ]
Moshage, Han [3 ,4 ]
机构：
[1] Univ Autonoma Aguascalientes, Basic Sci Ctr, Dept Morphol, Aguascalientes, Aguascalientes, Mexico
[2] Univ Groningen, Univ Med Ctr Groningen, Dept Gastroenterol & Hepatol, Groningen, Netherlands
[3] Univ Autonoma Aguascalientes, Basic Sci Ctr, Dept Physiol, Aguascalientes, Aguascalientes, Mexico
[4] Univ Groningen, Univ Med Ctr Groningen, Dept Lab Med, Groningen, Netherlands
来源：
JOURNAL OF FUNCTIONAL FOODS | 2022年 / 89卷
关键词：
Diclofenac; Hepatocyte; Apoptosis; Natural products; Antioxidants; ANTIOXIDANT PROPERTIES; OXIDATIVE STRESS; APOPTOSIS; BETALAINS; P53; INHIBITION; MECHANISMS; CHEMISTRY; ENZYMES; BETANIN;
D O I：
10.1016/j.jff.2022.104960
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
This study aimed to investigate whether Opuntia spp-extracts protect against diclofenac (DF)-induced hepatotoxicity. Rats were pretreated with Opuntia extracts, betanin (Bet) and N-acetylcysteine (NAC) followed by a single challenge of diclofenac. Liver tissue was collected for biochemical and histological analysis. Primary rat hepatocytes were treated with diclofenac (400 mu mol/L) with and without pretreatment with Opuntia extract. Apoptosis was measured by caspase-3 activity and necrosis by Sytox green staining. RNA was isolated, and realtime qPCR was performed to assess mRNA levels of stress and apoptosis-related genes MnSOD (SOD2), GADD45B and P53. ROS production was measured using the fluorescent MitoSOX assay. Results demonstrated that Opuntia spp-extracts protect against DF-induced liver toxicity via reducing oxidative stress and the inhibition of P53.
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页数：11
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