Nutrient associated changes in plasma membrane H+-ATPase activity of permeabilized Candida albicans cells

被引：0
|
作者：
Manzoor, N [1 ]
Rashid, B [1 ]
Amin, M [1 ]
Khan, LA [1 ]
机构：
[1] Jamia Millia Islamia, Ctr Biosci, New Delhi 110025, India
来源：
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS | 2000年 / 37卷 / 04期
关键词：
D O I：
暂无
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Nutrient stimulated H+ extrusion by cells and spheroplasts of Candida albicans and its underlying mechanism has been investigated. Glucose and glutamic acid stimulated extrusion by a factor of 6 and 1.4 respectively in cells, but had no effect on spheroplasts. Proline showed no H+ stimulation in cells, however, it stimulated spheroplasts 1.5 fold. ATPase activity of toluene-ethanol-Triton-X-100 (TET) permeabilized cells decreased by 20% following exposure to glucose and proline and 30% following exposure to glutamic acid. Permeabilized spheroplasts of Candida albicans showed drastic reduction in ATPase activity by 40%, 70% and 85% following exposure to glucose, proline and glutamic acid respectively. Externally added F-actin fully restored activity in the case of permeabilized cells and partly with spheroplasts suggesting that ATPase interacts with other membrane proteins during nutrient stimulation of cells.
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页码：241 / 244
页数：4
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