Occurrence and repair of alkylating stress in the intracellular pathogen Brucella abortus

被引:15
|
作者
Poncin, Katy [1 ,2 ]
Roba, Agnes [1 ]
Jimmidi, Ravikumar [3 ]
Potemberg, Georges [1 ]
Fioravanti, Antonella [4 ,5 ]
Francis, Nayla [1 ]
Willemart, Kevin [1 ]
Zeippen, Nicolas [1 ]
Machelart, Arnaud [1 ,6 ]
Biondi, Emanuele G. [4 ]
Muraille, Eric [7 ,8 ]
Vincent, Stephane P. [2 ]
De Bolle, Xavier [1 ]
机构
[1] Univ Namur, Narilis, URBM, Namur, Belgium
[2] Univ Oxford, Sir William Dunn Sch Pathol, South Parks Rd, Oxford OX1 3RE, England
[3] Univ Namur, Unite Chim Organ, 61 Rue Bruxelles, B-5000 Namur, Belgium
[4] Univ Lille, CNRS, UMR 8576, Unite Glycobiol Struct & Fonct, 50 Ave Halley, Villeneuve Dascq, France
[5] Vrije Univ Brussel, VIB, Pl Laan 2, B-1050 Brussels, Belgium
[6] Univ Lille, Ctr Infect & Immun Lille, CHU Lille, Inst Pasteur Lille,CNRS,INSERM,U1019,UMR 8204, Lille, France
[7] Aix Marseille Univ, IMM, 31 Chemin Joseph Aiguier, F-130091 Marseille, France
[8] Univ Libre Bruxelles, Fac Med, Lab Parasitol, Brussels, Belgium
关键词
N-NITROSO COMPOUNDS; DNA METHYLTRANSFERASE; ESCHERICHIA-COLI; ADAPTIVE RESPONSE; GENE-EXPRESSION; ALKB GENE; DAMAGE; CAULOBACTER; METHYLATION; MELITENSIS;
D O I
10.1038/s41467-019-12516-8
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
It is assumed that intracellular pathogenic bacteria have to cope with DNA alkylating stress within host cells. Here we use single-cell reporter systems to show that the pathogen Brucella abortus does encounter alkylating stress during the first hours of macrophage infection. Genes encoding direct repair and base-excision repair pathways are required by B. abortus to face this stress in vitro and in a mouse infection model. Among these genes, ogt is found to be under the control of the conserved cell-cycle transcription factor GcrA. Our results highlight that the control of DNA repair in B. abortus displays distinct features that are not present in model organisms such as Escherichia coli.
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页数:13
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