Akirin2 promotes slow myosin heavy chain expression by CaN/NFATc1 signaling in porcine skeletal muscle satellite cells

被引：6
|
作者：
Chen, Xiaoling [1 ]
Luo, Yanliu [1 ]
Huang, Zhiqing [1 ]
Liu, Guangmang [1 ]
Zhao, Hua [1 ]
机构：
[1] Sichuan Agr Univ, Key Lab Anim Dis Resistance Nutr, China Minist Educ, Inst Anim Nutr, Chengdu 611130, Sichuan, Peoples R China
来源：
ONCOTARGET | 2017年 / 8卷 / 15期
基金：
中国国家自然科学基金;
关键词：
Akirin2; slow myosin heavy chain; CaN/NFATc1; porcine skeletal muscle satellite cells; FIBER-TYPE; GENE-EXPRESSION; MEAT QUALITY; INTRAMUSCULAR FAT; PIG BREEDS; PROTEINS; IDENTIFICATION; PATHWAY;
D O I：
10.18632/oncotarget.15374
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
The objective of this study was to evaluate the effect of Akirin2 on slow myosin heavy chain (slow MyHC, MyHC I) gene expression and its molecular mechanisms. In this study, we showed that the protein expression of Akirin2 in pig slow oxidative Psoas major muscle is higher than that in fast glycolytic tibialis anterior muscle, suggesting that Akirin2 may play a role in myofiber typing. Knockdown of Akirin2 decreased the MyHC I expression and the calcineurin (CaN) activity, and also decreased the expressions of NFATc1 and MCIP1.4. Conversely, overexpression of Akirin2 got the opposite results. Furthermore, inhibition of CaN or knockdown of NFATc1 attenuated Akirin2 overexpression-induced upregulation of MyHC I. Together, these results demonstrate that Akirin2 promotes MyHC I expression via CaN/NFATc1 signaling pathway in porcine skeletal muscle satellite cells.
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页码：25158 / 25166
页数：9
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