False-positive rifampicin resistance on Xpert® MTB/RIF caused by a silent mutation in the rpoB gene

被引：45
|
作者：
Mathys, V. [1 ]
van de Vyvere, M. [2 ]
de Droogh, E. [3 ]
Soetaert, K. [1 ]
Groenen, G. [4 ]
机构：
[1] Sci Inst Publ Hlth, Natl Reference Ctr TB & Mycobacteria Communicable, Brussels, Belgium
[2] Ziekenhuis Netwerk Antwerpen ZNA Stuivenberg, Dept Microbiol, Antwerp, Belgium
[3] ZNA Middelheim, Dept Pneumol, Antwerp, Belgium
[4] Belgian Lung & TB Assoc, Brussels, Belgium
来源：
INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE | 2014年 / 18卷 / 10期
关键词：
tuberculosis; molecular test; gene sequencing; MYCOBACTERIUM-TUBERCULOSIS;
D O I：
10.5588/ijtld.14.0297
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
The Xpert (R) MTB/RIF assay detects the presence of Mycobacterium tuberculosis and its resistance to rifampicin (RMP) directly in sputum samples. Discrepant results were observed in a case of smear-positive pulmonary tuberculosis that was Xpert-resistant but phenotypically susceptible to RMP. Complementary investigations (repeat Xpert, Genotype (R) MTBDRplus assay and sequencing of the rpoB gene) revealed the presence of a silent mutation in the rpoB gene, leading to the conclusion of a false-positive Xpert result. As misinterpretation of Xpert results may lead to inappropriate treatment, the presence of rpoB mutations should be confirmed by sequencing the rpoB gene.
引用
收藏
页码：1255 / 1257
页数：3
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