PCR-restriction fragment length polymorphism assay for detection of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli

被引：20

作者：

Alonso, R ^{[1
]}

Mateo, E ^{[1
]}

Girbau, C ^{[1
]}

Churruca, E ^{[1
]}

Martinez, I ^{[1
]}

Fernández-Astorga, A ^{[1
]}

机构：

[1] Univ Basque Country, UPV EHU, Fac Farm, Dept Inmunol Microbiol & Parasitol, Vitoria 01006, Spain

来源：

ANTIMICROBIAL AGENTS AND CHEMOTHERAPY | 2004年 / 48卷 / 12期

关键词：

D O I：

10.1128/AAC.48.12.4886-4888.2004

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A fragment of the gyrA gene was sequenced from 34 isolates of Campylobacter coli, including 23 isolates resistant to ciprofloxacin. All ciprofloxacin-resistant isolates examined by DNA sequencing carried a point mutation at position Thr-86 on the gyrA gene product, involving the replacement of Thr-86 by Ile. A combined PCR-restriction fragment length polymorphism technique using RsaI was developed to detect this mutation.

引用

页码：4886 / 4888

页数：3

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