Methods to Evaluate the Activity of Nitric Oxide Synthase

被引：1
|
作者：
Fantacuzzi, Marialuigia [1 ]
Ammazzalorso, Alessandra [1 ]
De Filippis, Barbara [1 ]
Giampietro, Letizia [1 ]
Maccallini, Cristina [1 ]
Amoroso, Rosa [1 ]
机构：
[1] Univ G dAnnunzio, Dept Pharm, Chieti, Italy
来源：
CURRENT PHARMACEUTICAL ANALYSIS | 2017年 / 13卷 / 05期
关键词：
Nitric oxide synthase; nitric oxide; L-arginine; L-citrulline; griess assay; hemoglobin assay; citrulline assay; RP-HPLC assay; SELECTIVE INHIBITORS; GRIESS REACTION; PERMEABLE INHIBITORS; BLOOD-PRESSURE; POTENT; DESIGN; DERIVATIVES; TETRAHYDROBIOPTERIN; OPTIMIZATION; ACETAMIDINES;
D O I：
10.2174/1573412912666160916154405
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented. Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay. Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of L-citrulline as stoichiometric to nitric oxide. Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.
引用
收藏
页码：411 / 416
页数：6
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