Methods to Evaluate the Activity of Nitric Oxide Synthase

被引：1

作者：

Fantacuzzi, Marialuigia ^{[1
]}

Ammazzalorso, Alessandra ^{[1
]}

De Filippis, Barbara ^{[1
]}

Giampietro, Letizia ^{[1
]}

Maccallini, Cristina ^{[1
]}

Amoroso, Rosa ^{[1
]}

机构：

[1] Univ G dAnnunzio, Dept Pharm, Chieti, Italy

来源：

CURRENT PHARMACEUTICAL ANALYSIS | 2017年 / 13卷 / 05期

关键词：

Nitric oxide synthase; nitric oxide; L-arginine; L-citrulline; griess assay; hemoglobin assay; citrulline assay; RP-HPLC assay; SELECTIVE INHIBITORS; GRIESS REACTION; PERMEABLE INHIBITORS; BLOOD-PRESSURE; POTENT; DESIGN; DERIVATIVES; TETRAHYDROBIOPTERIN; OPTIMIZATION; ACETAMIDINES;

D O I：

10.2174/1573412912666160916154405

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Objective: The most used techniques to evaluate the inhibitor activity of different compounds on Nitric Oxide Synthases are presented. Method: Griess assay, oxy-hemoglobin capture assay, Radio citrulline assay, and RP-HPLC assay. Result: Griess assay, and oxy-hemoglobin capture assay detect the nitric oxide produced by NOS; while radio citrulline assay, and RP-HPLC assay take into consideration the concentration of L-citrulline as stoichiometric to nitric oxide. Conclusion: Griess assay and oxy-hemoglobin capture assay are suitable for a first screening of NOS activity, while the others could be used for a more precise activity determination.

引用

页码：411 / 416

页数：6

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