Dyella tabacisoli sp. nov., a bacterium isolated from an arable soil sample of Nicotiana tabacum L.

A Gram-stain-negative, aerobic, non-motile and rod-shaped bacterium, designated strain L4-6(T), was isolated from an arable soil sample of tobacco in Huize, south-western China and subjected to polyphasic taxonomic characterization. The cells showed oxidase-positive and catalase-positive reactions. Growth occurred at 20-35 degrees C, at pH 5.0-8.0 and with 0-2 % (w/v) NaCl, optimally at 30 degrees C, pH 6.0-7.0 and 0-1 % (w/v) NaCl. The major respiratory lipoquinone was ubiquinone-8. The predominant cellular fatty acids (>10.0 %) were identified as summed feature 9 (iso-C-17:1 omega 9c or C-16:0 10-methyl), iso-C-15:0 and iso-C-1(7:)0. The polar lipid profile contained diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, two unidentified phospholipids, two unidentified aminophospholipids and five unidentified aminolipids. The genomic DNA G+C content was 60.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain L4-6(T) should be affiliated to the genus Dyella and formed a Glade with the most closely related organism Dyella soli JS12-10(T). 16S rRNA gene sequences similarity analysis showed that strain L4-6(T) was mostly closely related to a soil JS12-10(T) (98.73 %) and Dyella lipolytica DHOBO7(T) (98.02 %). DNA-DNA hybridization data indicated that strain L4-6(T) represented a novel genomic species belonging to the genus Dyella. The polyphasic taxonomic characteristics indicated that the strain L4-6(T) represents a novel species of the genus Dyella, for which the name Dyella tabacisoli sp. nov. (type strain L4-6(T)=CGMCC 1.16273(T)=KCIC 62035(T)) is proposed.