High-resolution HLA-DQB1 typing using the polymerase chain reaction and sequence-specific primers

被引：19
|
作者：
Mullighan, CG [1 ]
Bunce, M [1 ]
Welsh, KI [1 ]
机构：
[1] Churchill Hosp, Tissue Typing Transplant Ctr, Oxford Transplant Ctr, Nuffield Dept Surg, Oxford OX3 7LJ, England
来源：
TISSUE ANTIGENS | 1997年 / 50卷 / 06期
关键词：
allele-specific typing; histocompatibility testing; HLA class II; HLA-DQB1; PCR-ARMS; PCR-SSP; transplantation;
D O I：
10.1111/j.1399-0039.1997.tb02936.x
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
Polymorphism at HLA-DQB1 is known to influence tissue compatibility and disease susceptibility; however, current DQB1 typing methods are unable to distinguish the 32 currently recognized DQB1 alleles. We have developed a 32-reaction PCR-SSP method capable of differentiating all DQB1 alleles that differ in amino acid sequence. This method can resolve all heterozygous combinations of DQB1 alleles, with the exception of several combinations involving alleles not thus far detected in Caucasoid populations.
引用
收藏
页码：688 / 692
页数：5
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