Quantification by additive RT-PCR of HIV-1 RNA plasma levels in different stages of HIV-1 infection

被引：0

作者：

Fernandez-Arcas, N ^{[1
]}

Dieguez-Lucena, JL ^{[1
]}

Costa, AF ^{[1
]}

Garcia-Villanova, J ^{[1
]}

Ruiz-Galdon, M ^{[1
]}

Pena, J ^{[1
]}

Morell, M ^{[1
]}

Reyes-Engel, A ^{[1
]}

机构：

[1] Univ Malaga, Fac Med, Dept Bioquim & Biol Mol, Malaga 29080, Spain

来源：

JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY | 1997年 / 53卷 / 03期

关键词：

quantitative PCR; HIV-disease;

D O I：

暂无

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In this study, virion-associated RNA was measured in plasma from twenty six patients in various stages of HIV-1 disease by the additive RT-PCR method. Plasma viral RNA levels were inversely correlated (r = -0.72894) with total CD4(+) cell counts and directly (r = 0.86964) with serum titre beta(2)-microglobulin in chronically infected patients. This additive RT-PCR is based on a mathematical logistic adjustment of the standard curve and the use of an internal standard identical to the target molecule, which represents a control system for the efficiency of RT-PCR and allows a continuous assessment of the accuracy based on the recovery.

引用

页码：307 / 315

页数：9

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