Intracellular imaging of organelles with new water-soluble benzophenoxazine dyes

被引：34

作者：

Jose, Jiney ^{[1
]}

Loudet, Aurore ^{[1
]}

Ueno, Yuichiro ^{[1
]}

Barhoumi, Rola ^{[2
]}

Burghardt, Robert C. ^{[2
]}

Burgess, Kevin ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Chem, College Stn, TX 77841 USA

[2] Texas A&M Univ, Dept Vet Integrat Biosci, College Stn, TX 77843 USA

来源：

ORGANIC & BIOMOLECULAR CHEMISTRY | 2010年 / 8卷 / 09期

基金：

美国国家卫生研究院;

关键词：

NILE RED; FLUORESCENT DYES; CYTOCHROME-C; RELEASE; MITOCHONDRIA; DEATH; CELLS;

D O I：

10.1039/b925845k

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

Five new water-soluble derivatives of Nile Red 1-5 were prepared. These benzophenoxazine dyes fluoresce between 640 and 667 nm with quantum yields of 0.17-0.33 in pH 7.4 phosphate buffer, and at slightly shorter wavelengths and higher quantum yields in EtOH. Two dyes, 3 and 4 permeated into Clone 9 cells and selectively stained mitochondria and golgi, respectively.

引用

页码：2052 / 2059

页数：8

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