Preparation of poly(γ-glutamic acid)/hydroxyapatite monolith via biomineralization for bone tissue engineering

被引：16
|
作者：
Park, Sung-Bin [1 ]
Hasegawa, Urara [1 ,2 ]
van der Vlies, Andre J. [1 ,3 ]
Sung, Moon-Hee [4 ,5 ]
Uyama, Hiroshi [1 ]
机构：
[1] Osaka Univ, Grad Sch Engn, Dept Appl Chem, Suita, Osaka 5650871, Japan
[2] Osaka Univ, Grad Sch Engn, Frontier Res Base Global Young Researchers, Suita, Osaka 5650871, Japan
[3] Osaka Univ, Frontier Res Ctr, Grad Sch Engn, Suita, Osaka 5650871, Japan
[4] Kookmin Univ, Dept Bio & Fermentat Convergence Technol, Seoul 136702, South Korea
[5] BioLeaders Corp, Taejon 305500, South Korea
来源：
JOURNAL OF BIOMATERIALS SCIENCE-POLYMER EDITION | 2014年 / 25卷 / 17期
基金：
日本学术振兴会;
关键词：
biomineralization; thermally induced phase separation; tissue engineering; monolith; poly(gamma-glutamic acid); hydroxyapatite; GAMMA-GLUTAMIC ACID; GROWTH-FACTOR; POLYMER SCAFFOLDS; PORE-SIZE; HYDROXYAPATITE; APATITE; FABRICATION; NANOCOMPOSITES; SIALOPROTEIN; ANGIOGENESIS;
D O I：
10.1080/09205063.2014.953404
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
A hybrid monolith of poly(gamma-glutamic acid) and hydroxyapatite (PGA/HAp monolith) was prepared via biomineralization and used as a macroporous cell scaffold in bone tissue engineering. The PGA monolith having a bimodal pore size distribution was used as a substrate to induce biomineralization. The PGA/HAp monolith was obtained by immersing the PGA monolith in simulated body fluid. Pretreatment with CaCl2 enhanced the apatite-forming ability of the PGA monolith. Murine osteoblastic MC3T3-E1 cells efficiently attached and proliferated on the PGA/HAp monolith. MTT assay showed that both the PGA and PGA/HAp monolith did not have apparent cytotoxicity. Moreover, the PGA and PGA/HAp monoliths adsorbed bone morphogenetic protein-2 (BMP-2) by electrostatic interaction which was slowly released in the medium during cell culture. The PGA/HAp monolith enhanced BMP-2 induced alkaline phosphatase activity compared to the PGA monolith and a polystyrene culture plate. Thus, these PGA/HAp monoliths may have potential in bone tissue engineering.
引用
收藏
页码：1875 / 1890
页数：16
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