Emergence of ST147 Klebsiella pneumoniae Producing OXA-204 Carbapenemase in a University Hospital, Tunisia

被引：22
|
作者：
Grami, Raoudha [1 ,2 ]
Mansour, Wejdene [2 ,3 ]
Khalifa, Anis Ben Haj [4 ]
Dahmen, Safia [3 ]
Chatre, Pierre [1 ]
Haenni, Marisa [1 ]
Aouni, Mahjoub [2 ]
Madec, Jean-Yves [1 ]
机构：
[1] ANSES Site Lyon, Unite Antibioresistance & Virulence Bacteriennes, Lyon, France
[2] Fac Pharm, Lab Maladies Transmissibles & Subst Biologiqement, Monastir, Tunisia
[3] Hop Univ Sahloul, Lab Microbiol, Unite Rech Resistances Bacteriennes Emergentes Se, Sousse 4054, Tunisia
[4] Hop Tahar Sfar, Lab Microbiol, Mahdia, Tunisia
来源：
MICROBIAL DRUG RESISTANCE | 2016年 / 22卷 / 02期
关键词：
D BETA-LACTAMASE; MULTIPLEX PCR; OXA-48; ENTEROBACTERIACEAE;
D O I：
10.1089/mdr.2014.0278
中图分类号：
R51 [传染病];
学科分类号：
100401 ;
摘要：
Molecular features of the first carbapenem-resistant Klebsiella pneumoniae isolates (KP1 and KP2) from the University Hospital Tahar Sfar, Tunisia, were investigated. Antimicrobial susceptibility testing, multilocus sequence typing, S1 nuclease pulsed-field gel electrophoresis, Southern blot, and polymerase chain reaction (PCR)-based replicon typing were performed. Extended-spectrum beta-lactamases and carbapenemase genes were detected by PCR and sequencing. Both isolates were multidrug resistant. KP1 was of sequence type (ST) ST101 and exhibited bla(CTX-M-15) and bla(TEM-1) on an untypeable plasmid and bla(OXA-48) on an IncL/M plasmid. KP2 was genetically unrelated to KP1 (ST147) and harbored an IncA/C plasmid carrying bla(CMY-4) and the bla(OXA-48) derivative gene: bla(OXA-204). This study reports the second case worldwide of an OXA-204-producing K. pneumoniae isolate from the same country, however, in a different genetic background.
引用
收藏
页码：137 / 140
页数：4
相关论文
共 50 条
  • [11] Emergence and rapid dissemination of highly resistant NDM-14-producing Klebsiella pneumoniae ST147, France, 2022
    Emeraud, Cecile
    Mahamat, Aba
    Jousset, Agnes B.
    Bernabeu, Sandrine
    Goncalves, Tania
    Pommier, Camille
    Girlich, Delphine
    Birer, Aurelien
    Rodriguez, Christophe
    Pawlotsky, Jean-Michel
    Naas, Thierry
    Bonnin, Remy A.
    Dorte, Laurent
    EUROSURVEILLANCE, 2023, 28 (42)
  • [12] Structure and gene cluster of the capsular polysaccharide of multidrug resistant carbapenemase OXA-48-producing Klebsiella pneumoniae strain KPB536 of the genetic line ST147
    Volozhantsev, Nikolay V.
    Shpirt, Anna M.
    Kislichkina, Angelina A.
    Shashkov, Alexander S.
    Verevkin, Vladimir V.
    Fursova, Nadezhda K.
    Knirel, Yuriy A.
    RESEARCH IN MICROBIOLOGY, 2020, 171 (02) : 74 - 79
  • [13] ST147 NDM-1-producing Klebsiella pneumoniae spread in two Tunisian hospitals
    Messaoudi, Aziza
    Haenni, Marisa
    Mansour, Wejdene
    Saras, Estelle
    Khalifa, Anis Bel Haj
    Chaouch, Cherifa
    Naija, Walid
    Boujaafar, Noureddine
    Bouallegue, Olfa
    Madec, Jean-Yves
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2017, 72 (01) : 315 - 316
  • [14] Emergence of NDM-4 and OXA-181 carbapenemase-producing Klebsiella pneumoniae
    Kim, Jin Seok
    Hong, Chae-Kyu
    Park, Sang-Hun
    Jin, Young-Hee
    Han, Sunghee
    Kim, Hee Soon
    Park, Joo-Hyun
    Bae, Byung-Noe
    Chung, Ju-Young
    Han, Tae-Hee
    Lee, Jib-Ho
    Lee, Sang-Me
    Oh, Young-Hee
    JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 2020, 20 : 332 - 333
  • [15] Emergence of OXA-48 carbapenemase-producing Escherichia coli and Klebsiella pneumoniae in dogs
    Stolle, Inka
    Prenger-Berninghoff, Ellen
    Stamm, Ivonne
    Scheufen, Sandra
    Hassdenteufel, Esther
    Guenther, Sebastian
    Bethe, Astrid
    Pfeifer, Yvonne
    Ewers, Christa
    JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 2013, 68 (12) : 2802 - 2808
  • [16] Emergence of Klebsiella pneumoniae Carbapenemase-Producing Bacteria
    Arnold, Ryan S.
    Thom, Kerri A.
    Sharma, Saarika
    Phillips, Michael
    Johnson, J. Kristie
    Morgan, Daniel J.
    SOUTHERN MEDICAL JOURNAL, 2011, 104 (01) : 40 - 45
  • [17] Clonal Dissemination of KPC-2, VIM-1, OXA-48-Producing Klebsiella pneumoniae ST147 in Katowice, Poland
    Ochonska, Dorota
    Klaminska-Cebula, Hanna
    Dobrut, Anna
    Bulanda, Malgorzata
    Brzychczy-Wloch, Monika
    POLISH JOURNAL OF MICROBIOLOGY, 2021, 70 (01) : 107 - 116
  • [18] Challenges of controlling a large outbreak of OXA-48 carbapenemase-producing Klebsiella pneumoniae in a French university hospital
    Semin-Pelletier, B.
    Cazet, L.
    Bourigault, C.
    Juvin, M. E.
    Boutoille, D.
    Raffi, F.
    Hourmant, M.
    Blancho, G.
    Agard, C.
    Connault, J.
    Corvec, S.
    Caillon, J.
    Batard, E.
    Lepelletier, D.
    JOURNAL OF HOSPITAL INFECTION, 2015, 89 (04) : 248 - 253
  • [19] Klebsiella pneumoniae carbapenemase-producing Serratia marcescens outbreak in a university hospital
    Tuon, Felipe Francisco
    Cordova, Kassia
    Dario, Thayrine Mayara
    Nunes, Luciana de Souza
    Barth, Afonso Luis
    Martins, Andreza Francisco
    AMERICAN JOURNAL OF INFECTION CONTROL, 2017, 45 (06) : 700 - 702
  • [20] Epidemiology of Klebsiella pneumoniae carbapenemase (KPC) producing Klebsiella pneumoniae in a German hospital
    Wendt, C.
    Schuett, S.
    Dalpke, A.
    Zimmermann, S.
    Trierweiler-Hauke, B.
    Konrad, M.
    Mieth, M.
    Weigand, M.
    Hoppe-Tichy, T.
    Jonas, D.
    INTERNATIONAL JOURNAL OF MEDICAL MICROBIOLOGY, 2008, 298 : 34 - 35
12345