Molecular characterization of serogrouping and virulence genes of Malaysian Vibrio cholerae isolated from different sources

被引：12
|
作者：
Teh, Cindy Shuan Ju [1 ]
Thong, Kwai Lin [1 ]
Ngoi, Soo Tein [1 ]
Ahmad, Norazah [2 ]
Nair, Gopinath Balakrish [3 ]
Ramamurthy, Thandavarayan [3 ]
机构：
[1] Univ Malaya, Inst Biol Sci, Fac Sci, Kuala Lumpur 50603, Malaysia
[2] Inst Med Res, Div Bacteriol, Kuala Lumpur 50588, Malaysia
[3] Natl Inst Cholera & Enter Dis, Kolkata, India
来源：
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY | 2009年 / 55卷 / 06期
关键词：
multiplex PCR; serogroup; Vibrio cholerae; virulence gene; MULTIPLEX PCR; STRAINS; IDENTIFICATION; DIVERSITY; ASSAY; O1;
D O I：
10.2323/jgam.55.419
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
A pair of primers targeting the hlyA gene for Vibrio cholerae which could distinguish the classical from El Tor biotypes was designed and combined with other specific primers for ompW, rfb complex, and virulence genes such as ctxA, toxR, and tcpl in a multiplex PCR (m-PCR) assay. This m-PCR correctly identified 39 V cholerae from clinical, water and seafood samples. The efficiency of this multiplex PCR (m-PCR) was compared with conventional biochemical and sero-grouping methods. One 0139 and 25 01 V cholerae strains including 10 environmental strains harbored all virulence-associated genes except 1 clinical strain which only had toxR and hlyA genes. Thirteen environmental strains were classified as non-O1/non-O139 and had the toxR and hlyA genes only. The detection limit of m-PCR was 7 x 10(4) cfu/ml. The m-PCR test was reliable and rapid and reduced the identification time to 4 h.
引用
收藏
页码：419 / 425
页数：7
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