Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

被引：22
|
作者：
Nishikawa, Fumie [1 ]
Endo, Tomoko [1 ]
Shimada, Takehiko [1 ]
Fujii, Hiroshi [1 ]
Shimizu, Tokurou [1 ]
Kobayashi, Yasushi [2 ]
Araki, Takashi [3 ]
Omura, Mitsuo [4 ]
机构：
[1] Natl Agr Res Org, Natl Inst Fruit Tree Sci, Okitsu Citrus Res Stn, Shimizu ku, Shizuoka 4240292, Japan
[2] Max Planck Inst Dev Biol, D-72076 Tubingen, Germany
[3] Kyoto Univ, Grad Sch Biostudies, Div Integrated Life Sci, Sakyo Ku, Kyoto 6068501, Japan
[4] Shizuoka Univ, Fac Agr, Dept Biol Sci, Shizuoka 4228529, Japan
来源：
TREE PHYSIOLOGY | 2010年 / 30卷 / 03期
关键词：
citrus; flowering; Flowering Locus T (FT); FRUITFULL (FUL); SEPALLATA (SEP); ARABIDOPSIS FRUIT-DEVELOPMENT; LOCUS-T PROTEIN; FLORAL INDUCTION; FT PROTEIN; LONG-DISTANCE; CONSTITUTIVE EXPRESSION; SATSUMA MANDARIN; INTEGRATOR FT; CITRUS; GENES;
D O I：
10.1093/treephys/tpp122
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using a 22-K oligo DNA microarray. Microarray results and reverse transcription polymerase chain reaction analysis indicated that genes relating to transcription, cell wall modification and defense responses were expressed at higher levels in the transgenic shoots than in the control wild-type shoots. Of the genes showing ectopic mRNA accumulation, two SEPALLATA (SEP) and one FRUITFULL (FUL) homologues (CuSEP1, CuSEP3 or CuFUL) were introduced to Arabidopsis thaliana. Constitutive expression of each gene caused early flowering in transgenic Arabidopsis, suggesting that these genes could function as regulators of flowering time.
引用
下载
收藏
页码：431 / 439
页数：9
相关论文
共 50 条
  • [21] Boron toxicity induced specific changes of cell ultrastructure and architecture of components in leaf center and tip of trifoliate orange [Poncirus trifoliata (L.) Raf.]
    Wu, Xiuwen
    Lu, Xiaopei
    Riaz, Muhammad
    Yan, Lei
    Jiang, Cuncang
    JOURNAL OF ENVIRONMENTAL MANAGEMENT, 2019, 246 : 426 - 433
  • [22] Ectopic expression of an FT homolog from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.)
    Endo, T
    Shimada, T
    Fujii, H
    Kobayashi, Y
    Araki, T
    Omura, M
    TRANSGENIC RESEARCH, 2005, 14 (05) : 703 - 712
  • [23] Ectopic Expression of an FT Homolog from Citrus Confers an Early Flowering Phenotype on Trifoliate Orange (Poncirus trifoliata L. Raf.)
    Tomoko Endo
    Takehiko Shimada
    Hiroshi Fujii
    Yasushi Kobayashi
    Takashi Araki
    Mitsuo Omura
    Transgenic Research, 2005, 14 : 703 - 712
  • [24] High frequency shoot regeneration from trifoliate orange (Poncirus trifoliata L. Raf.) using the thin cell layer method
    Van Le, B
    Ha, NT
    Hong, LTA
    Vân, KTT
    COMPTES RENDUS DE L ACADEMIE DES SCIENCES SERIE III-SCIENCES DE LA VIE-LIFE SCIENCES, 1999, 322 (12): : 1105 - 1111
  • [25] CRYOPRESERVATION OF EMBRYONIC AXES OF TRIFOLIATE ORANGE (PONCIRUS-TRIFOLIATA [L] RAF)
    RADHAMANI, J
    CHANDEL, KPS
    PLANT CELL REPORTS, 1992, 11 (04) : 204 - 206
  • [26] Copia-like retrotransposons in a precocious mutant of trifoliate orange [Poncirus trifoliata (L.) Raf]
    Tao, N. G.
    Wei, J.
    Liu, Y. Z.
    Cheng, Y. J.
    Deng, X. X.
    JOURNAL OF HORTICULTURAL SCIENCE & BIOTECHNOLOGY, 2006, 81 (06): : 1038 - 1042
  • [27] 枳Poncirus　trifoliata（L．）Raf．的种质资源
    郭天池
    陈全友
    叶荫民
    杜宜秀
    中国南方果树, 1996, (03) : 8 - 10
  • [28] Arbuscular mycorrhizal fungi can alter some root characters and physiological status in trifoliate orange (Poncirus trifoliata L. Raf.) seedlings
    Wu, Qiang-Sheng
    Zou, Ying-Ning
    He, Xin-Hua
    Luo, Peng
    PLANT GROWTH REGULATION, 2011, 65 (02) : 273 - 278
  • [29] Arbuscular mycorrhizal fungi can alter some root characters and physiological status in trifoliate orange (Poncirus trifoliata L. Raf.) seedlings
    Qiang-Sheng Wu
    Ying-Ning Zou
    Xin-Hua He
    Peng Luo
    Plant Growth Regulation, 2011, 65 : 273 - 278
  • [30] Influence of nutrient deficiency on root architecture and root hair morphology of trifoliate orange (Poncirus trifoliata L. Raf.) seedlings under sand culture
    Cao, Xiu
    Chen, Chunli
    Zhang, Dejian
    Shu, Bo
    Xiao, Jie
    Xia, Renxue
    SCIENTIA HORTICULTURAE, 2013, 162 : 100 - 105
12345