Transcriptional changes in CiFT-introduced transgenic trifoliate orange (Poncirus trifoliata L. Raf.)

被引：22
|
作者：
Nishikawa, Fumie [1 ]
Endo, Tomoko [1 ]
Shimada, Takehiko [1 ]
Fujii, Hiroshi [1 ]
Shimizu, Tokurou [1 ]
Kobayashi, Yasushi [2 ]
Araki, Takashi [3 ]
Omura, Mitsuo [4 ]
机构：
[1] Natl Agr Res Org, Natl Inst Fruit Tree Sci, Okitsu Citrus Res Stn, Shimizu ku, Shizuoka 4240292, Japan
[2] Max Planck Inst Dev Biol, D-72076 Tubingen, Germany
[3] Kyoto Univ, Grad Sch Biostudies, Div Integrated Life Sci, Sakyo Ku, Kyoto 6068501, Japan
[4] Shizuoka Univ, Fac Agr, Dept Biol Sci, Shizuoka 4228529, Japan
来源：
TREE PHYSIOLOGY | 2010年 / 30卷 / 03期
关键词：
citrus; flowering; Flowering Locus T (FT); FRUITFULL (FUL); SEPALLATA (SEP); ARABIDOPSIS FRUIT-DEVELOPMENT; LOCUS-T PROTEIN; FLORAL INDUCTION; FT PROTEIN; LONG-DISTANCE; CONSTITUTIVE EXPRESSION; SATSUMA MANDARIN; INTEGRATOR FT; CITRUS; GENES;
D O I：
10.1093/treephys/tpp122
中图分类号：
S7 [林业];
学科分类号：
0829 ; 0907 ;
摘要：
Ectopic expression of a Flowering Locus T (CiFT) from Citrus confers an early flowering phenotype on trifoliate orange (Poncirus trifoliata L. Raf.). To profile transcriptional effects of CiFT, mRNA extracted from the shoots of the transgenic trifoliate orange was subjected to genetic analysis using a 22-K oligo DNA microarray. Microarray results and reverse transcription polymerase chain reaction analysis indicated that genes relating to transcription, cell wall modification and defense responses were expressed at higher levels in the transgenic shoots than in the control wild-type shoots. Of the genes showing ectopic mRNA accumulation, two SEPALLATA (SEP) and one FRUITFULL (FUL) homologues (CuSEP1, CuSEP3 or CuFUL) were introduced to Arabidopsis thaliana. Constitutive expression of each gene caused early flowering in transgenic Arabidopsis, suggesting that these genes could function as regulators of flowering time.
引用
收藏
页码：431 / 439
页数：9
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