Investigation of Seizure-Susceptibility in a Drosophila melanogaster Model of Human Epilepsy with Optogenetic Stimulation

被引：11
|
作者：
Saras, Arunesh [1 ]
Wu, Veronica V. [1 ]
Brawer, Harlan J. [1 ]
Tanouye, Mark A. [1 ,2 ]
机构：
[1] Univ Calif Berkeley, Div Organismal Biol, Dept Environm Sci Policy & Management, Berkeley, CA 94720 USA
[2] Univ Calif Berkeley, Dept Mol & Cell Biol, Berkeley, CA 94720 USA
来源：
GENETICS | 2017年 / 206卷 / 04期
基金：
美国国家卫生研究院;
关键词：
sodium channel; epilepsy; seizure-suppression; red light activable channelrhodopsin; SENSITIVE PARALYTIC MUTANTS; ONSET EPISODIC ATAXIA; SODIUM-CHANNEL GENE; MUSHROOM BODY; NEONATAL EPILEPSY; OLFACTORY MEMORY; NEURAL CIRCUITS; BEHAVIOR; ACTIVATION; NEURONS;
D O I：
10.1534/genetics.116.194779
中图分类号：
Q3 [遗传学];
学科分类号：
071007 ; 090102 ;
摘要：
We examined seizure-susceptibility in a Drosophila model of human epilepsy using optogenetic stimulation of ReaChR (red-activatable channelrhodopsin). Photostimulation of the seizure-sensitive mutant para(bss1) causes behavioral paralysis that resembles paralysis caused by mechanical stimulation, in many aspects. Electrophysiology shows that photostimulation evokes abnormal seizure-like neuronal firing in para(bss1) followed by a quiescent period resembling synaptic failure and apparently responsible for paralysis. The pattern of neuronal activity concludes with seizure-like activity just prior to recovery. We tentatively identify the mushroom body as one apparent locus of optogenetic seizure initiation. The alpha/beta lobes may be primarily responsible for mushroom body seizure induction.
引用
收藏
页码：1739 / 1746
页数：8
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