Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction

被引：50

作者：

Cox-Singh, J ^{[1
]}

Mahayet, S ^{[1
]}

Abdullah, MS ^{[1
]}

Singh, B ^{[1
]}

机构：

[1] Univ Sci Malaysia, Sch Med Sci, Kelantan, Malaysia

来源：

INTERNATIONAL JOURNAL FOR PARASITOLOGY | 1997年 / 27卷 / 12期

关键词：

malaria; PCR; field epidemiology; Plasmodium;

D O I：

10.1016/S0020-7519(97)00147-1

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Malaria remains a disease of underdeveloped and remote regions of the world. The application of polymerase chain reaction (PCR) technology to malaria epidemiology has the potential for increasing our knowledge and understanding of this disease. In order to study malaria in all geographical locations it is important that specimen collection and DNA extraction for PCR be kept simple. Here we report a method for extracting DNA from dried blood spots on filter paper which is capable of detecting one Plasmodium falciparum and two Plasmodium vivax parasites/mu l of whole blood by nested PCR without compromising the simplicity of specimen collection or DNA extraction. (C) 1997 Australian Society for Parasitology. Published by Elsevier Science Ltd.

引用

页码：1575 / 1577

页数：3

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