Identification of Extrachromosomal Circular DNA in Hop via Rolling Circle Amplification

被引：10
|
作者：
Diaz-Lara, Alfredo [1 ]
Gent, David H. [2 ]
Martin, Robert R. [3 ]
机构：
[1] Oregon State Univ, Dept Bot & Plant Pathol, 2082 Cordley Hall,2701 SW Campus Way, Corvallis, OR 97331 USA
[2] USDA ARS, Forage Seed & Cereal Res Lab, Corvallis, OR USA
[3] USDA ARS, Hort Crops Res Lab, Corvallis, OR USA
来源：
CYTOGENETIC AND GENOME RESEARCH | 2016年 / 148卷 / 2-3期
关键词：
Circular DNA; Extrachromosomal DNA; Hop; Rolling circle amplification; SATELLITE REPEATS; TANDEM REPEATS; EUKARYOTES; MICRODNAS; PLASMID; PLANTS;
D O I：
10.1159/000445849
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
During a survey for new viruses affecting hop plants, a circular DNA molecule was identified via rolling circle amplification (RCA) and later characterized. A small region of the 5.7-kb long molecule aligned with a microsatellite region in the Humulus lupulus genome, and no coding sequence was identified. Sequence analysis and literature review suggest that the small DNA molecule is an extranuclear DNA element, specifically, an extrachromosomal circular DNA (eccDNA), and its presence was confirmed by electron microscopy. This work is the first report of eccDNAs in the family Cannabaceae. Additionally, this work highlights the advantages of using RCA to study extrachromosomal DNA in higher plants. (C) 2016 S. Karger AG, Basel
引用
收藏
页码：237 / 240
页数：4
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