A Protein Microsensor by Detection of Leakage Current from Interaction between an Electrolyte-Entrapping Liposome and Protein

被引：2
|
作者：
Noda, M. [1 ]
Asai, T. [1 ]
Shimanouchi, T. [2 ]
Yamashita, K. [1 ,2 ]
Umakoshi, H. [2 ]
Okuyama, M. [2 ]
Kuboi, R. [2 ]
机构：
[1] Kyoto Inst Technol, Grad Sch Sc & Tech, Sakyo Ku, Kyoto 6068585, Japan
[2] Osaka Univ, Grad Sch Engn Sci, Toyonaka, Osaka 5608531, Japan
来源：
PROCEEDINGS OF THE EUROSENSORS XXIII CONFERENCE | 2009年 / 1卷 / 01期
关键词：
Leakage current; Liposome; Electrolyte; Protein; Interaction; STRESS CONDITIONS; SENSOR SYSTEM;
D O I：
10.1016/j.proche.2009.07.323
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
We have newly made a specific biomolecule microsensor measuring leakage current and impedance with minute liposome solution that interacts with biomolecules such as protein. The microsensor was fabricated with a Si surface-bulk-micromachining process. K-4[Fe(CN)(6)] solution was entrapped inside of liposome of 1,2-dipalmitoyl-sn-glycero-3-phosphocholline (DPPC) and Carbonic Anhydrase from Bovine (CAB) was used as added protein for the interaction. A clear leakage in nA order was observed for the CAB, indicating the microsensor detects the CAB through the liposome-protein interaction. The detectivity increased by about 7 times and the volume of solution was decreased by about 10,000 from the previous result.
引用
收藏
页码：1295 / +
页数：2
相关论文
共 50 条
  • [1] A Leakage Current Microsensor for Detection of Interaction between an Electrolyte-Entrapping Liposome and Protein
    Noda, Minoru
    Asai, Takeshi
    Yamashita, Kaoru
    Shimanouchi, Toshinori
    Umakoshi, Hiroshi
    Okuyama, Masanori
    Kuboi, Ryouichi
    2009 IEEE SENSORS, VOLS 1-3, 2009, : 1883 - +
  • [2] New Planar-Type Leakage Current and Impedance Microsensor for Detection of Interaction between Electrolyte-Entrapping Liposome and Protein
    Lorchirachoonkul, P.
    Goto, I.
    Shimanouchi, T.
    Yamashita, K.
    Noda, M.
    EUROSENSORS XXV, 2011, 25
  • [3] A Highly-Sensitive Leakage Current Microsensor by Using Denaturant: Detection of Target Protein by DPPC Liposome
    Lorchirachoonkul, P.
    Shimanouchi, T.
    Yamashita, K.
    Umakoshi, H.
    Kuboi, R.
    Noda, M.
    EUROSENSORS XXIV CONFERENCE, 2010, 5 : 812 - 815
  • [4] Sensitivity Enhancement of Leakage Current Microsensor for Detection of Target Protein by Using Protein Denaturant
    Lorchirachoonkul, P.
    Yamashita, K.
    Noda, M.
    Shimanouchi, T.
    Umakoshi, H.
    Kuboi, R.
    2010 IEEE SENSORS, 2010, : 1056 - 1059
  • [5] Sensitivity Enhancement of Leakage Current Microsensor for Detection of Target Protein by Using Protein Denaturant
    Noda, Minoru
    Lorchirachoonkul, Prinya
    Shimanouchi, Toshinori
    Yamashita, Kaoru
    Umakoshi, Hiroshi
    Kuboi, Ryouichi
    IEEE SENSORS JOURNAL, 2011, 11 (11) : 2749 - 2755
  • [6] Evaluation of interaction between liposome membranes induced by stimuli responsive polymer and protein
    Félix, MM
    Umakoshi, H
    Shimanouchi, T
    Yoshimoto, M
    Kuboi, R
    JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2002, 93 (05) : 498 - 501
  • [7] The interaction mechanism between liposome and whey protein: Effect of liposomal vesicles concentration
    Chen, Yang
    Yi, Xiangzhou
    Pan, Min-Hsiung
    Chiou, Yi-Shiou
    Li, Zhenshun
    Wei, Shudong
    Yin, Xiaoli
    Ding, Baomiao
    JOURNAL OF FOOD SCIENCE, 2021, 86 (06) : 2491 - 2498
  • [8] The van der Waals interaction between protein molecules in an electrolyte solution
    Song, XY
    Zhao, XF
    JOURNAL OF CHEMICAL PHYSICS, 2004, 120 (04): : 2005 - 2009
  • [9] Protein-Protein Interaction Detection By SVM From Sequence Information
    Liu, Hong-Wei
    OPTIMIZATION AND SYSTEMS BIOLOGY, 2009, 11 : 198 - 206
  • [10] Interaction between liposome and myofibrillar protein in surimi: Effect on gel structure and digestive characteristics
    Yi, Xiangzhou
    Pei, Zhisheng
    Xia, Guanghua
    Liu, Zhongyuan
    Shi, Haohao
    Shen, Xuanri
    INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2023, 253
12345