Methylome profiling identifies TCHH methylation in CfDNA as a noninvasive marker of liver metastasis in colorectal cancer

被引：4
|
作者：
Chen, Shanwen [1 ]
Liu, Tao [1 ]
Bu, Dingfang [2 ]
Zhu, Jing [1 ]
Wang, Xin [1 ]
Pan, Yisheng [1 ]
Liu, Yucun [1 ]
Lu, Zhi John [3 ]
Wang, Pengyuan [1 ]
机构：
[1] Peking Univ First Hosp, Div Gen Surg, Beijing, Peoples R China
[2] Peking Univ First Hosp, Cent Lab, Beijing, Peoples R China
[3] Tsinghua Univ, Ctr Synthet & Syst Biol, Sch Life Sci, MOE Key Lab Bioinformat, Beijing, Peoples R China
来源：
FASEB JOURNAL | 2021年 / 35卷 / 07期
基金：
中国国家自然科学基金;
关键词：
CfDNA; colorectal cancer; liver metastasis; methylight; methylome; TCHH; DNA; PLASMA;
D O I：
10.1096/fj.202100266R
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Methylation of circulating free DNA (CfDNA) has emerged as an efficient marker of tumor screening and prognostics. However, no efficient methylation marker has been developed for monitoring liver metastasis (LM) in colorectal cancer (CRC). Utilizing methylome profiling and bisulfite sequencing polymerase chain reaction of paired primary and LM sites, significantly increased methylation of TCHH was identified in the process of LM in CRC in the present study. Methylight analysis of TCHH methylation in CfDNA displayed a promisingly discriminative power between CRC with and without LM. Besides, significant coefficient of TCHH methylation and LM tumor volume was also validated. Together, these results indicated the potential of TCHH methylation in CfDNA as a monitoring marker of LM in CRC.
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页数：11
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