Deletion of the intracellular domain of coxsackie and adenovirus receptor (CAR) enhances the expression of itself and boosts the efficiency of current adenovirus-mediated gene therapy in ovarian cancer cell lines in vitro

被引：4

作者：

Wang, Beibei ^{[1
]}

Chen, Gang ^{[1
]}

Zhou, Jianfeng ^{[1
]}

Wu, Peng ^{[1
]}

Luo, Danfeng ^{[1
]}

Huang, Xiaoyuan ^{[1
]}

Zhu, Tao ^{[1
]}

Han, Zhiqiang ^{[1
]}

Xu, Gang ^{[1
]}

Wang, Shixuan ^{[1
]}

Lu, Yunping ^{[1
]}

Ma, Ding ^{[1
]}

机构：

[1] Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Canc Biol Ctr, Hubei 430030, Peoples R China

来源：

CANCER LETTERS | 2007年 / 248卷 / 02期

基金：

中国国家自然科学基金;

关键词：

coxsackie and adenovirus receptor (CAR); gene therapy; ovarian cancer;

D O I：

10.1016/j.canlet.2006.08.002

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

The failure of adenovirus-mediated gene therapy often derives from the absence of coxsackie and adenovirus receptor (CAR) expression in target cells. We hypothesize that the slight up-regulation of CAR expression might boost the effect of adenovirus-mediated gene therapy in ovarian cancer. To test this hypothesis, we transfected full-length and intracellular-domain-deleted (tailless) CAR plasmids into CAR-deficient ovarian cancer cell line SKOV3. We observed significant elevations of the in vitro killing effect of Adv-TK and oncolytic adenovirus-mediated cytopathic effect (CPE) in transfected sub-clones, and tailless-transfected SKOV3 showed higher CAR expressions than full-length CAR-transfected cells. We conclude that the extracellular domain of CAR is essential for adenovirus-based gene therapy and, furthermore, that its intracellular domain might play an important role in the regulation of its own expression. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

引用

页码：299 / 307

页数：9

共 36 条

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L H Stockwin
J Eaton
I A Clarke
A G Dalgleishm
S M Todryk
G E Blair
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