Characterization of novel neopullulanase from Bacillus polymyxa

被引：13
|
作者：
Yebra, MJ [1 ]
Arroyo, J [1 ]
Sanz, P [1 ]
Prieto, JA [1 ]
机构：
[1] CSIC,INST AGROQUIM & TECNOL ALIMENTOS,BURJASSOT 46100,SPAIN
来源：
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 1997年 / 68卷 / 1-2期
关键词：
extracellular enzyme; neopullulanase; panose; pullulan; Bacillus polymyxa;
D O I：
10.1007/BF02785984
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacillus polymyxa CECT 155 produces an extracellular neopullulanase activity that degrades pullulan to panose. This activity was stimulated by the presence of pullulan in the culture, and repressed by glucose. The apparent mot wt determined for the enzyme was 58 kDa. The optimum pH and temperature for neopullulanase activity were pH 6.0 and 50 degrees C, respectively. The enzyme was stable in a pH range of 4.0-8.0, and temperatures up to 60 degrees C. These properties make it suitable for the saccharification processes in the starch industries.
引用
收藏
页码：113 / 120
页数：8
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