Surface Free Energy and Bacterial Attachment on Microtextured Ti6Al4V Alloy

The present study investigated the variation in surface free energy, adhesion strength, and bacterial attachment during early colonization. Two different sizes of micro dimple textured surface MDS 400 and MDS 200 on Ti6Al4V were fabricated using mechanical micro-milling and studied with an un-textured surface. One polar (water) and dispersive liquid (diiodomethane) were used to study the work of adhesion and surface free energy of both surfaces. The Neumann method and Owen & Wendt approach were used to calculate the SFE. EDS analysis was carried out to check the surface composition. E. coli bacterial strain was used to study the early colonization responses using FESEM and optical microscope. Results indicate that the formed textures are uniform with good quality and as per the design of experiment. Area surface roughness values of the textured surface have been increased from UTS. Besides, no tool wear debris has been found on textured surfaces. SFE and adhesion strength of water and diiodomethane increase due to surface texturing and initial colonization of bacteria on textured surfaces is in the form of E. coli cellular chains has been observed. The current study offers insights into the surface free energy, adhesion strength of water and diiodomethane, and bacterial attachment during the early stage of colonization.