Kinetics of the Transfer of Ubiquitin from UbcH7 to E6AP

被引：16

作者：

Purbeck, Carrie ^{[1
]}

Eletr, Ziad M. ^{[1
]}

Kuhlman, Brian ^{[1
]}

机构：

[1] Univ N Carolina, Dept Biochem & Biophys, Chapel Hill, NC 27599 USA

来源：

BIOCHEMISTRY | 2010年 / 49卷 / 07期

关键词：

HECT FAMILY; LIGASE; DETERMINANTS; SPECIFICITY;

D O I：

10.1021/bi9014693

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Prior to substrate ubiquitination by HECT-E3 ligases, ubiquitin must first be activated by El and then transferred via a series of transthiolation reactions from El to E2 and from E2 to E3. We have measured the rate constants and binding affinities underlying the transfer of ubiquitin from E2 UbcH7 to the HECT domain of E3 E6AP. We show that charged UbcH7 and free UbcH7 bind E6AP with similar affinities and that at 37 degrees C the second-order rate constant for the reaction (k(cat)/K-m) equals similar to 2.3 x 10(5) M-1 s(-1). The measured parameters place limits on substrate-E6AP binding lifetimes required for processive polyubiquitination.

引用

页码：1361 / 1363

页数：3

共 50 条

[41] Kinetic analysis of the interactions of human papillomavirus E6 oncoproteins with the ubiquitin ligase E6AP using surface plasmon resonance
Zanier, K
Charbonnier, S
Baltzinger, M
Nominé, Y
Altschuh, D
Travé, G
JOURNAL OF MOLECULAR BIOLOGY, 2005, 349 (02) : 401 - 412
[42] Folding and misfolding of the papillomavirus E6 interacting peptide E6ap
Cui, BX
Shen, MY
Freed, KF
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (12) : 7087 - 7092
[43] The ubiquitin ligase Triad1 inhibits myelopoiesis through UbcH7 and Ubc13 interacting domains
J A Marteijn
L T van der Meer
J J Smit
S M Noordermeer
W Wissink
P Jansen
H G Swarts
R G Hibbert
T de Witte
T K Sixma
J H Jansen
B A van der Reijden
Leukemia, 2009, 23 : 1480 - 1489
[44] The stability of the human papillomavirus E6 oncoprotein is E6AP dependent
Tomaic, Vjekoslav
Pim, David
Banks, Lawrence
VIROLOGY, 2009, 393 (01) : 7 - 10
[45] E6AP Promotes a Metastatic Phenotype in Prostate Cancer
Gamell, Cristina
Bandilovska, Ivona
Gulati, Twishi
Kogan, Arielle
Lim, Syer Choon
Kovacevic, Zaklina
Takano, Elena A.
Timpone, Clelia
Agupitan, Arjelle D.
Litchfield, Cassandra
Blandino, Giovanni
Horvath, Lisa G.
Fox, Stephen B.
Williams, Scott G.
Russo, Andrea
Gallo, Enzo
Paul, Piotr J.
Mitchell, Catherine
Sandhu, Shahneen
Keam, Simon P.
Haupt, Sue
Richardson, Des R.
Haupt, Ygal
ISCIENCE, 2019, 22 : 1 - +
[46] Proteomic identification of E6AP as a molecular target of tamoxifen in MCF7 cells
Lochab, Savita
Pal, Pooja
Kanaujiya, Jitendra K.
Tripathi, Shashi B.
Kapoor, Isha
Bhatt, Madan L. B.
Sanyal, Sabyasachi
Behre, Gerhard
Trivedi, Arun K.
PROTEOMICS, 2012, 12 (09) : 1363 - 1377
[47] The ubiquitin-protein ligase E6AP/UBE3A supports early encephalomyocarditis virus replication
Carmody, Marybeth
Zimmer, Joshua T.
Cushman, Camille H.
Thao Nguyen
Lawson, T. Glen
VIRUS RESEARCH, 2018, 252 : 48 - 57
[48] E6AP promotes the degradation of the PML tumor suppressor
I Louria-Hayon
O Alsheich-Bartok
Y Levav-Cohen
I Silberman
M Berger
T Grossman
K Matentzoglu
Y-H Jiang
S Muller
M Scheffner
S Haupt
Y Haupt
Cell Death & Differentiation, 2009, 16 : 1156 - 1166
[49] E6AP promotes the degradation of the PML tumor suppressor
Louria-Hayon, I.
Alsheich-Bartok, O.
Levav-Cohen, Y.
Silberman, I.
Berger, M.
Grossman, T.
Matentzoglu, K.
Jiang, Y-H
Muller, S.
Scheffner, M.
Haupt, S.
Haupt, Y.
CELL DEATH AND DIFFERENTIATION, 2009, 16 (08): : 1156 - 1166
[50] Human scribble (Vartul) is targeted for ubiquitin-mediated degradation by the high-risk papillomavirus E6 proteins and the E6AP ubiquitin-protein ligase
Nakagawa, S
Huibregtse, JM
MOLECULAR AND CELLULAR BIOLOGY, 2000, 20 (21) : 8244 - 8253

← 1 2 3 4 5 →