Identification and characterization of a novel elastase inhibitor from Hirudinaria manillensis

被引：6
|
作者：
Xu Kuan-Hong [1 ]
Zhou Meng [3 ]
Wu Fei-Long [2 ,4 ]
Tang Xiao-Peng [2 ]
Lu Qiu-Min [2 ,4 ]
Lai Ren [2 ,4 ]
Long Cheng-Bo [2 ,4 ]
机构：
[1] Univ Sci & Technol China, Sch Life Sci, Hefei 230027, Peoples R China
[2] Chinese Acad Sci, Kunming Inst Zool, Key Lab Bioact Peptides Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming 650223, Yunnan, Peoples R China
[3] Chongqing Inst Med Plant Cultivat, Nanchuan 408435, Peoples R China
[4] Univ Chinese Acad Sci, Beijing 100049, Peoples R China
来源：
CHINESE JOURNAL OF NATURAL MEDICINES | 2021年 / 19卷 / 07期
基金：
美国国家科学基金会;
关键词：
Elastase inhibitor; Hirudinaria manillensis; HMEI-A; NET formation; Anti-inflammation; NEUTROPHIL EXTRACELLULAR TRAPS; AMINO-ACID-SEQUENCE; THROMBIN INHIBITOR; MEDICINAL LEECH;
D O I：
10.1016/S1875-5364(21)60054-7
中图分类号：
R [医药、卫生];
学科分类号：
10 ;
摘要：
A large number of protease inhibitors have been found from leeches, which are essential in various physiological and biological processes. In the curret study, a novel elastase inhibitor was purified and characterized from the leech of Hirudinaria manillensis, which was named HMEI-A. Primary structure analysis showed that HMEI-A belonged to a new family of proteins. HMEI-A exerted inhibitory effects on elastase and showed potent abilities to inhibit elastase with an inhibition constant (K-i) of 1.69 x 10(-8) mol.L-1. Further study showed that HMEI-A inhibited the formation of neutrophil extracellular trap (NET). These results suggested that HMEI-A from the leech of H. manillensis is a novel elastase inhibitor which can suppress NET formation. It may play a significant role in blood-sucking of leeches and is a potential candidate as an anti-inflammatory agent.
引用
收藏
页码：540 / 544
页数：5
相关论文
共 50 条
  • [31] CORE DOMAIN OF HIRUDIN FROM THE LEECH HIRUDINARIA-MANILLENSIS - CHEMICAL SYNTHESIS, PURIFICATION, AND CHARACTERIZATION OF A TRP(3) ANALOG OF FRAGMENT 1-47
    DEFILIPPIS, V
    VINDIGNI, A
    ALTICHIERI, L
    FONTANA, A
    BIOCHEMISTRY, 1995, 34 (29) : 9552 - 9564
  • [32] Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa
    Shining Loo
    Antony Kam
    Tianshu Xiao
    Giang K. T. Nguyen
    Chuan Fa Liu
    James P. Tam
    Scientific Reports, 6
  • [33] Identification and Characterization of Roseltide, a Knottin-type Neutrophil Elastase Inhibitor Derived from Hibiscus sabdariffa
    Loo, Shining
    Kam, Antony
    Xiao, Tianshu
    Nguyen, Giang K. T.
    Liu, Chuan Fa
    Tam, James P.
    SCIENTIFIC REPORTS, 2016, 6
  • [34] PURIFICATION AND CHARACTERIZATION OF AN ELASTASE INHIBITOR DERIVED FROM HYALINE CARTILAGE
    DIMUZIO, MT
    TRIPIER, D
    KUETTNER, KE
    JOURNAL OF RHEUMATOLOGY, 1987, 14 : 45 - 48
  • [35] The core domain of hirudin from the leech Hirudinaria manillensis - Chemical modification of a tryptophan-containing synthetic peptide analog
    DeAntoni, F
    DeFilippis, V
    Altichieri, L
    Vindigni, A
    deLaureto, PP
    Fontana, A
    RECENT ADVANCES IN TRYPTOPHAN RESEARCH: TRYPTOPHAN AND SEROTONIN PATHWAYS, 1996, 398 : 627 - 633
  • [36] Identification and structural characterization of a novel myeloperoxidase inhibitor from Staphylococcus delphini
    Ploscariu, Nicoleta T.
    de Jong, Nienke W. M.
    van Kessel, Kok P. M.
    van Strijp, Jos A. G.
    Geisbrecht, Brian V.
    ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2018, 645 : 1 - 11
  • [37] ELASTASE INHIBITOR - CHARACTERIZATION OF THE HUMAN ELASTASE INHIBITOR MOLECULE ASSOCIATED WITH MONOCYTES, MACROPHAGES, AND NEUTROPHILS
    REMOLDODONNELL, E
    NIXON, JC
    ROSE, RM
    JOURNAL OF EXPERIMENTAL MEDICINE, 1989, 169 (03): : 1071 - 1086
  • [38] STRUCTURE OF ELASNIN, A NOVEL ELASTASE INHIBITOR
    OMURA, S
    NAKAGAWA, A
    OHNO, H
    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1979, 101 (15) : 4386 - 4388
  • [39] AZD9668: Pharmacological Characterization of a Novel Oral Inhibitor of Neutrophil Elastase
    Stevens, T.
    Ekholm, K.
    Granse, M.
    Lindahl, M.
    Kozma, V.
    Jungar, C.
    Ottosson, T.
    Falk-Hakansson, H.
    Churg, A.
    Wright, J. L.
    Lal, H.
    Sanfridson, A.
    JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS, 2011, 339 (01): : 313 - 320
  • [40] Identification and characterization of novel sirtuin inhibitor scaffolds
    Sanders, Brandi D.
    Jackson, Brittany
    Brent, Michael
    Taylor, Alexander M.
    Dang, Weiwei
    Berger, Shelley L.
    Schreiber, Stuart L.
    Howitz, Konrad
    Marmorstein, Ronen
    BIOORGANIC & MEDICINAL CHEMISTRY, 2009, 17 (19) : 7031 - 7041
12345