ESR measurement using 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO) in human erythrocyte ghosts

被引：5
|
作者：
Shioji, Kosei [1 ]
Iwashita, Hidefumi [1 ]
Shimomura, Taiji [1 ]
Yamaguchi, Takeo [1 ]
Okuma, Kentaro [1 ]
机构：
[1] Fukuoka Univ, Fac Sci, Dept Chem, Jonan Ku, Fukuoka 8140180, Japan
来源：
BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN | 2007年 / 80卷 / 04期
关键词：
D O I：
10.1246/bcsj.80.758
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Competitive spin-trapping behavior of 2-diphenylphosphinoyl-2-methyl-3,4-dihydro-2H-pyrrole N-oxide (DPhPMPO) and 2,2-dimethyl-3,4-dihydro-2H-pyrrole N-oxide (DMPO) indicated that the affinity of DPhPMPO for hydroxyl radicals was 1.7 times higher than that of DMPO. Human erythrocyte ghosts (HEGs) with encapsulated Feil catalysts (HEGFe) were prepared for ESR measurement. By trapping the hydroxyl radical (OH.) in the presence of polyethylene glycol (PEG-4000) outside the HEG-Fe, the generation of OH. inside the catalyst was confirmed. The trapping of hydroxyl radicals generated in HEG was accomplished using DPhPMPO, and the permeability of the erythrocyte membrane to DPhPMPO was 2.4 times greater than that of DMPO.
引用
收藏
页码：758 / 762
页数：5
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