Comparison between plasma and whole blood specimens for detection of Aspergillus DNA by PCR

被引：82
|
作者：
Loeffler, J
Hebart, H
Brauchle, U
Schumacher, U
Einsele, H
机构：
[1] Univ Tubingen, Med Klin, Abt 2, Lab Prof Dr Med H Einsele, D-72076 Tubingen, Germany
[2] Univ Tubingen, Inst Hyg, D-72076 Tubingen, Germany
来源：
JOURNAL OF CLINICAL MICROBIOLOGY | 2000年 / 38卷 / 10期
关键词：
D O I：
10.1128/JCM.38.10.3830-3833.2000
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Ninety-six plasma and whole blood specimens from nine selected patients were analyzed for the presence of Aspergillus DNA. Nineteen specimens from three patients with proven aspergillosis mere PCR positive in both materials, whereas an additional 22 were PCR positive in whole blood only, All 36 samples from six patients without signs of aspergillosis were negative in both assays. We conclude that although plasma and whole blood spiked with Aspergillus conidia shelved an identical lower detection limit (10 CFU), the sensitivity of plasma PCR was lower than that of PCR performed on whole blood samples.
引用
收藏
页码：3830 / 3833
页数：4
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