An optimized protocol for the retroviral transduction of mouse CD4 T cells

被引:4
|
作者
Eremenko, Ekaterina [1 ,2 ,3 ,4 ,5 ]
Taylor, Zoe, V [1 ,2 ,4 ,5 ]
Khand, Bishnu [1 ,2 ,4 ,5 ]
Zaccai, Shir [1 ,2 ,3 ,4 ,5 ]
Porgador, Angel [1 ,2 ,4 ,5 ]
Monsonego, Alon [1 ,2 ,4 ,5 ]
机构
[1] Bengurion Univ Negev, Fac Hlth Sci, Shraga Segal Dept Microbiol Immunol & Genet, IL-8410501 Beer Sheva, Israel
[2] Bengurion Univ Negev, Fac Hlth Sci, Dept Physiol & Cell Biol, IL-8410501 Beer Sheva, Israel
[3] Bengurion Univ Negev, Dept Life Sci, IL-8410501 Beer Sheva, Israel
[4] Bengurion Univ Negev, Natl Inst Biotechnol Negev, Zlotowski Neurosci Ctr, IL-8410501 Beer Sheva, Israel
[5] Bengurion Univ Negev, Regenerat Med & Stem Cell Res Ctr, IL-8410501 Beer Sheva, Israel
来源
STAR PROTOCOLS | 2021年 / 2卷 / 03期
关键词
IL-7;
D O I
10.1016/j.xpro.2021.100719
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.
引用
收藏
页数:13
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