An optimized protocol for the retroviral transduction of mouse CD4 T cells

被引：4

作者：

Eremenko, Ekaterina ^{[1
,2
,3
,4
,5
]}

Taylor, Zoe, V ^{[1
,2
,4
,5
]}

Khand, Bishnu ^{[1
,2
,4
,5
]}

Zaccai, Shir ^{[1
,2
,3
,4
,5
]}

Porgador, Angel ^{[1
,2
,4
,5
]}

Monsonego, Alon ^{[1
,2
,4
,5
]}

机构：

[1] Bengurion Univ Negev, Fac Hlth Sci, Shraga Segal Dept Microbiol Immunol & Genet, IL-8410501 Beer Sheva, Israel

[2] Bengurion Univ Negev, Fac Hlth Sci, Dept Physiol & Cell Biol, IL-8410501 Beer Sheva, Israel

[3] Bengurion Univ Negev, Dept Life Sci, IL-8410501 Beer Sheva, Israel

[4] Bengurion Univ Negev, Natl Inst Biotechnol Negev, Zlotowski Neurosci Ctr, IL-8410501 Beer Sheva, Israel

[5] Bengurion Univ Negev, Regenerat Med & Stem Cell Res Ctr, IL-8410501 Beer Sheva, Israel

来源：

STAR PROTOCOLS | 2021年 / 2卷 / 03期

关键词：

IL-7;

D O I：

10.1016/j.xpro.2021.100719

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Transduction of primary T cells has become prominent with the introduction of chimeric antigen receptor T-cell therapy. Although there are many protocols for the transduction of human T cells, it remains a challenge to transduce murine T cells. We present an optimized protocol for the retroviral transduction of murine CD4 T cells, which overcomes major challenges including large-scale production and long-term culturing of transduced cells. The optimized protocol combines high transduction efficiency with a low rate of cell death.For complete details on the use and execution of this protocol, please refer to Eremenko et al., 2019.

引用

页数：13

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