Evaluating the impact of hydroxychloroquine on mouse lymphocyte proliferation and cytokine production in vivo and in vitro

被引：1
|
作者：
Wabitsch, Simon [1 ]
McCallen, Justin D. [1 ]
Ma, Chi [1 ]
Greten, Tim F. [1 ]
机构：
[1] NCI, Gastrointestinal Malignancy Sect, Thorac & GI Malignancies Branch, Ctr Canc Res,NIH, 9000 Rockville Pike,10-3B43, Bethesda, MD 20892 USA
来源：
STAR PROTOCOLS | 2021年 / 2卷 / 02期
关键词：
Cancer; Flow Cytometry/Mass Cytometry; Immunology;
D O I：
10.1016/j.xpro.2021.100517
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Immunomodulatory drugs can alter lymphocyte function. Hydroxychloroquine (HCQ) is prescribed for many autoimmune diseases and is under investigation as an anti-tumor autophagy inhibitor. Here, we describe a protocol to evaluate the influence of HCQ on lymphocyte function by measuring the in vitro and ex vivo proliferation and cytokine production. The protocol can provide insights into potential immunomodulatory effects of HCQ and can be used for assessing other medications' effects on lymphocyte functions.For complete details on the use and execution of this protocol, please refer to Wabitsch et al. (2021).
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页数：10
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