Ammonium and phosphate limitation in 1,3-propanediol production by Klebsiella pneumoniae

被引：10
|
作者：
Zheng, Zong-Ming [1 ]
Xu, Yun-Zhen [2 ]
Wang, Ti-Peng [1 ]
Dong, Chang-Qing [1 ]
Yang, Yong-Ping [1 ]
Liu, De-Hua [2 ]
机构：
[1] N China Elect Univ, Sch Renewable Energy, Natl Engn Lab Power Generat Equipment, Beijing 102206, Peoples R China
[2] Tsinghua Univ, Dept Chem Engn, Beijing 100084, Peoples R China
来源：
BIOTECHNOLOGY LETTERS | 2010年 / 32卷 / 02期
关键词：
Ammonium limitation; Fermentation; Klebsiella pneumoniae; Phosphate limitation; 1,3-Propanediol; MICROBIAL-GROWTH; FERMENTATION; GLYCEROL; SUBSTRATE; CULTURE; PATHWAY;
D O I：
10.1007/s10529-009-0150-y
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
Excretion of 1,3-propanediol (1,3-PD) by K. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-PD in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. Ammonia-limited fed-batch cultures produced 61 g 1,3-PD l(-1) and a total of 15 g l(-1) organic acid in 36 h. However, phosphate-limited fed-batch cultures excreted 61 g lactate l(-1) and 44 g 1,3-PD l(-1).
引用
收藏
页码：289 / 294
页数：6
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