Genetic characterization of sarcophagid flies by Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR)

被引：0
|
作者：
Bajpai, Neelam [1 ]
Tewari, R. R. [1 ]
机构：
[1] Univ Allahabad, Dept Zool, Allahabad 211002, Uttar Pradesh, India
来源：
NATIONAL ACADEMY SCIENCE LETTERS-INDIA | 2010年 / 33卷 / 3-4期
关键词：
RAPD-PCR; flesh flies; genetic characterization; Sarcophaga; ARBITRARY PRIMERS; IDENTIFICATION; DIPTERA; MARKERS; CULICIDAE; PROVINCE; CHINA;
D O I：
暂无
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Two species of flesh flies belonging to the genus Sarcophaga, namely, S. albiceps and S. knabi were genetically characterized using Random Amplified Polymorphic DNA-Polymerase Chain Reaction (RAPD-PCR). In S. knabi and S. albiceps 21 and 20 fragments were amplified ranging from 196 bp to 1500 bp, respectively. Thirteen exclusive fragments were found in both the species. Nei's genetic identity value (0.561), calculated using Tools For Population Genetic Analysis (TFPGA) software, reveals close genetic similarity between the two species.
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页码：103 / 106
页数：4
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