An electrochemiluminescence assay for sensitive detection of methyltransferase activity in different cancer cells by hybridization chain reaction coupled with a G-quadruplex/hemin DNAzyme biosensing strategy

被引：25

作者：

Zhang, Hui ^{[1
]}

Guo, Zhihui ^{[1
]}

Dong, Huilei ^{[1
]}

Chen, Hongfei ^{[1
]}

Cai, Chenxin ^{[1
]}

机构：

[1] Nanjing Normal Univ, Jiangsu Collaborat Innovat Ctr Biomed Funct Mat, Jiangsu Key Lab New Power Batteries,Coll Chem & M, Jiangsu Key Lab Biomed Mat,Natl & Local Joint Eng, Nanjing 210097, Jiangsu, Peoples R China

来源：

ANALYST | 2017年 / 142卷 / 11期

关键词：

DNA METHYLATION; SIGNAL AMPLIFICATION; RESTRICTION-ENDONUCLEASE; COLORIMETRIC ASSAY; ELECTROCHEMICAL DETECTION; DNMT1; METHYLTRANSFERASE; GOLD NANOPARTICLES; CLEAVAGE; TUMORS; PROBE;

D O I：

10.1039/c7an00486a

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

In this work, a highly sensitive electrochemiluminescence (ECL) assay was fabricated for the detection of human DNA (cytosine-5)-methyltransferase1 (DNMT1) activity in cancer cells. The ECL assay coupled hybridization chain reaction with a G-quadruplex/hemin DNAzyme biosensing strategy. The ECL intensity changes (Delta I) allowed detection of DNMT1 activity down to 0.09 U mL(-1), and.I was proportional to the logarithm of the activity of DNMT1 within the range of 1.0 to 30.0 U mL(-1) in buffer solution. It also showed high sensitivity to DNMT1 activity in A549 cells, with a detection limit of about 2 cells. This ECL assay provides a promising platform for profiling of the mutational cells of tumors and shows a great potential for application to DNA methylation-related clinical diagnostics.

引用

页码：2013 / 2019

页数：7

共 23 条

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