Recombinant proteins containing amino acid sequences of two ectatomin chains

被引：0

作者：

Esipov, RS ^{[1
]}

Gurevich, AI ^{[1
]}

Kayushin, AL ^{[1
]}

Korosteleva, MD ^{[1
]}

Miroshnikov, AI ^{[1
]}

Shevchenko, LV ^{[1
]}

Pluzhnikov, KA ^{[1
]}

Grishin, EV ^{[1
]}

机构：

[1] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Moscow 117871, Russia

来源：

BIOORGANICHESKAYA KHIMIYA | 1997年 / 23卷 / 12期

关键词：

ectatomin; recombinant proteins; genes; chemical and enzymic synthesis;

D O I：

暂无

中图分类号：

学科分类号：

摘要：

Artificial genes for chains A and B of ectatomin, an Ectatomma tuberculatum ant toxin, were obtained by chemical and enzymic synthesis and cloned into new plasmid vectors. Expression plasmids with the genes of hybrid proteins were constructed containing human interleukin-3 or its terminal 63-mer fragment as well as chains A and B of ectatomin, which are linked via a region containing the cleavage site of specific protease, enterokinase (hybrid proteins IL3ETOXA, IL3ETOXB, ILETOXA, and ILETOXB). Escherichia coli producer strains providing a high yield of IL3ETOXA and IL3ETOXB proteins as inclusion bodies were obtained.

引用

页码：949 / 952

页数：4

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