High-level expression of a soluble snake venom enzyme, gloshedobin, in E-coli in the presence of metal ions

被引：26

作者：

Yang, Q ^{[1
]}

Xu, JQ ^{[1
]}

Li, M ^{[1
]}

Lei, XY ^{[1
]}

An, LJ ^{[1
]}

机构：

[1] Dalian Univ Technol, Bioengn Inst, Dalian 116012, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2003年 / 25卷 / 08期

关键词：

Escherichia coli; expression; gloshedobin; thrombin-like enzyme;

D O I：

10.1023/A:1023067626846

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The mature gene of gloshedobin, a snake venom thrombin-like enzyme from the snake, Gloydius shedaoensis, was cloned and expressed in strain E. coli BL21(DE3). Having been induced by IPTG, the recombinant gloshedobin was in both soluble and insoluble forms. To avoid inclusion body formation, expression was optimized at 25degreesC. Furthermore, a 50% increase in solubilization of the target protein was obtained by adding 0.1 mM Mg2+ to the medium. The purified recombinant gloshedobin gave a 44 kDa band on SDS-PAGE gel.

引用

页码：607 / 610

页数：4

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