Heterogeneous vascular permeability and alternative diffusion barrier in sensory circumventricular organs of adult mouse brain

被引:59
|
作者
Morita, Shoko [1 ,2 ]
Furube, Eriko [1 ]
Mannari, Tetsuya [1 ]
Okuda, Hiroaki [2 ]
Tatsumi, Kouko [2 ]
Wanaka, Akio [2 ]
Miyata, Seiji [1 ]
机构
[1] Kyoto Inst Technol, Dept Appl Biol, Sakyo Ku, Kyoto 6068585, Japan
[2] Nara Med Univ, Dept Anat & Neurosci, Fac Med, Kashihara, Nara 6348521, Japan
关键词
Blood-brain barrier; Tight junction; Vascular permeability; Astrocyte; Perivascular space; Mouse; TIGHT JUNCTION PROTEINS; AREA POSTREMA; MEDIATED DISRUPTION; MONOCLONAL-ANTIBODY; SUBFORNICAL ORGAN; RAT-BRAIN; EXPRESSION; ANGIOGENESIS; CADHERIN; OCCLUDIN;
D O I
10.1007/s00441-015-2207-7
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Fenestrated capillaries of the sensory circumventricular organs (CVOs), including the organum vasculosum of the lamina terminalis, the subfornical organ and the area postrema, lack completeness of the blood-brain barrier (BBB) to sense a variety of blood-derived molecules and to convey the information into other brain regions. We examine the vascular permeability of blood-derived molecules and the expression of tight-junction proteins in sensory CVOs. The present tracer assays revealed that blood-derived dextran 10 k (Dex10k) having a molecular weight (MW) of 10,000 remained in the perivascular space between the inner and outer basement membranes, but fluorescein isothiocyanate (FITC; MW: 389) and Dex3k (MW: 3000) diffused into the parenchyma. The vascular permeability of FITC was higher at central subdivisions than at distal subdivisions. Neither FITC nor Dex3k diffused beyond the dense network of glial fibrillar acidic protein (GFAP)-positive astrocytes/tanycytes. The expression of tight-junction proteins such as occludin, claudin-5 and zonula occludens-1 (ZO-1) was undetectable at the central subdivisions of the sensory CVOs but some was expressed at the distal subdivisions. Electron microscopic observation showed that capillaries were surrounded with numerous layers of astrocyte processes and dendrites. The expression of occludin and ZO-1 was also observed as puncta on GFAP-positive astrocytes/tanycytes of the sensory CVOs. Our study thus demonstrates the heterogeneity of vascular permeability and expression of tight-junction proteins and indicates that the outer basement membrane and dense astrocyte/tanycyte connection are possible alternative mechanisms for a diffusion barrier of blood-derived molecules, instead of the BBB.
引用
收藏
页码:497 / 511
页数:15
相关论文
共 50 条
  • [41] Blood-brain barrier permeability in the periventricular areas of the normal mouse brain
    M. Ueno
    I. Akiguchi
    M. Hosokawa
    H. Kotani
    K. Kanenishi
    H. Sakamoto
    Acta Neuropathologica, 2000, 99 : 385 - 392
  • [42] Blood-brain barrier permeability in the periventricular areas of the normal mouse brain
    Ueno, M
    Akiguchi, I
    Hosokawa, M
    Kotani, H
    Kanenishi, K
    Sakamoto, H
    ACTA NEUROPATHOLOGICA, 2000, 99 (04) : 385 - 392
  • [43] Blood-Brain Barrier Permeability Abnormalities in Vascular Cognitive Impairment
    Taheri, Saeid
    Gasparovic, Charles
    Huisa, Branko N.
    Adair, John C.
    Edmonds, Elaine
    Prestopnik, Jillian
    Grossetete, Mark
    Shah, N. Jon
    Wills, John
    Qualls, Clifford
    Rosenberg, Gary A.
    STROKE, 2011, 42 (08) : 2158 - U232
  • [44] Distribution of neurogranin-like immunoreactivity in the brain and sensory organs of the adult zebrafish
    Alba-Gonzalez, Anabel
    Folgueira, Monica
    Castro, Antonio
    Anadon, Ramon
    Yanez, Julian
    JOURNAL OF COMPARATIVE NEUROLOGY, 2022, 530 (10) : 1569 - 1587
  • [45] Activation of microglia and macrophages in the circumventricular organs of the mouse brain during TLR2-induced fever and sickness responses
    Murayama, Saki
    Kurganov, Erkin
    Miyata, Seiji
    JOURNAL OF NEUROIMMUNOLOGY, 2019, 334
  • [46] Expression pattern of mUBPy in the brain and sensory organs of mouse during embryonic development
    d'Amora, Marta
    Angelini, Cristiano
    Aluigi, Maria Grazia
    Marcoli, Manuela
    Maura, Guido
    Berruti, Giovanna
    Vallarino, Mauro
    BRAIN RESEARCH, 2010, 1355 : 16 - 30
  • [47] Glucocorticoid effects on mouse microvascular endothelial barrier permeability are brain specific
    Foerster, Carola
    Waschke, Jens
    Burek, Malgorzata
    Leers, Jorg
    Drenckhahn, Detlev
    JOURNAL OF PHYSIOLOGY-LONDON, 2006, 573 (02): : 413 - 425
  • [48] Assessment of Blood–Brain Barrier Permeability Using the In Situ Mouse Brain Perfusion Technique
    Rong Zhao
    J. Cory Kalvass
    Gary M. Pollack
    Pharmaceutical Research, 2009, 26
  • [49] Effect of mobile telephony on blood-brain barrier permeability in the fetal mouse brain
    Finnie, JW
    Blumbergs, PC
    Cai, Z
    Manavis, J
    Kuchel, TR
    PATHOLOGY, 2006, 38 (01) : 63 - 65
  • [50] Astrocytes are necessary for blood-brain barrier maintenance in the adult mouse brain
    Heithoff, Benjamin P.
    George, Kijana K.
    Phares, Aubrey N.
    Zuidhoek, Ivan A.
    Munoz-Ballester, Carmen
    Robel, Stefanie
    GLIA, 2021, 69 (02) : 436 - 472