Usutu virus in wild birds in northern Italy

被引：84
|
作者：
Manarolla, G. [1 ]
Bakonyi, T. [2 ,3 ]
Gallazzi, D. [1 ]
Crosta, L. [4 ]
Weissenboeck, H. [5 ]
Dorrestein, G. M. [6 ]
Nowotny, N. [2 ]
机构：
[1] Univ Milan, Dipartimento Patol Anim Igiene & Sanita Pubbl Vet, I-20133 Milan, Italy
[2] Univ Vet Med Vienna, Dept Pathobiol, Zoonoses & Emerging Infect Grp, A-1210 Vienna, Austria
[3] Szent Istvan Univ, Dept Microbiol & Infect Dis, Fac Vet Sci, H-1143 Budapest, Hungary
[4] Ctr Serv Vet Valcurone SRL, I-23873 Missaglia, LC, Italy
[5] Univ Vet Med Vienna, Dept Pathobiol, Inst Pathol & Forens Vet Med, A-1210 Vienna, Austria
[6] NOIVBD, Diagnost Pathol Lab, NL-5507 PP Veldhoven, Netherlands
来源：
VETERINARY MICROBIOLOGY | 2010年 / 141卷 / 1-2期
关键词：
Usutu virus; Wild birds; Immunohistochemistry; RT-PCR; Sequencing; INFECTIONS; EMERGENCE; AUSTRIA;
D O I：
10.1016/j.vetmic.2009.07.036
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Usutu virus (USUV) infection was diagnosed in two free-living blackbirds and in three captive owls belonging to two different species in northern Italy in the summers of 2006-2008. Diagnosis was established by immunohistochemistry and RT-PCR. RT-PCR was performed on frozen and on paraffin-embedded tissues (PET), respectively. From the frozen samples a partial sequence of the putative USUV E and NS1 proteins (1229 bp) was determined, whereas partial sequences of the putative NS3 (278 bp) and NS5 (159 bp) proteins were obtained from PET. Additionally, one partial sequence (163 bp) of the putative 3'UTR region was determined from all samples. Sequencing of the amplification products revealed 99.8-100% nucleotide identity of the Italian USUV strains to those from other central European countries. (C) 2009 Elsevier B.V. All rights reserved.
引用
收藏
页码：159 / 163
页数：5
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